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使用黄金纳米颗粒与1,9-二甲基甲蓝相结合的抗菌光动力疗法,用于治疗葡萄球菌皮肤感染

Anildo Alves de Brito Júnior¹, Pedro Jorge Louro Crugeira¹, Wellington Luis Reis Costa¹

¹Federal University of Bahia, Salvador, Brazil.

Lasers in medical science

|February 19, 2025

概括

使用DMMB-AuNPs和红色LED光的抗微生物光动力疗法 (aPDT) 有效地消除了大鼠伤口中的金黄色细菌感染. 这种新的方法实现了5-log的微生物减少,显示出显著的杀菌效应.

科学领域:

生物医学工程生物医学工程
摄影化学的使用.
纳米技术纳米技术

背景情况:

黄金葡萄球菌 (S. aureus) 感染在伤口护理中构成了重大挑战.
抗菌素耐药性需要新的治疗策略.
光动力疗法 (PDT) 提供了一种光激活的抗菌方法.

研究的目的:

评估抗微生物光动力疗法 (aPDT) 的有效性,使用西光敏感剂结合黄金纳米粒子 (DMMB-AuNPs) 治疗黄金菌的伤口感染.
评估DMMB-AuNPs和红色LED照射在老鼠伤口模型中的协同效应.

主要方法:

使用DMMB和5nm金纳米粒子制备DMMB-AuNP.
在体内研究涉及S. aureus污染的背部伤口在Wistar大鼠,分为四组 (控制,LED,DMMB-AuNPs,DMMB-AuNPs + LED).
红色LED光线 (12 J/cm2, λ630±20 nm) 的应用对相关群体.
使用殖民地形成单位 (CFU/mL) 量化S. aureus特异性媒介上的细菌负载.

主要成果:

与对照组 (p < 0.0001) 相比,aPDT组 (DMMB-AuNPs + LED) 显示了99.999%的显著微生物减少 (5日志减少).
在aPDT治疗组中观察到明显的杀菌作用.
在仅接受DMMB-AuNPs或LED光线的组中没有发现显著的减少.

结论:

通过DMMB-AuNPs介导的aPDT是一种高度有效的治疗S. aureus污染的伤口.
这种组合疗法是治疗葡萄球菌感染的有希望的替代方案.
这项研究强调了基于纳米结合物的aPDT在抗击细菌感染方面的潜力.

关键词:

黄金葡萄球菌黄金葡萄球菌纳米技术纳米技术摄影动力学无活化伤口上的伤口.

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