优化MEP残留物的清洁程序:来自新型HPLC-MS分析的见解
Mei-Ling Zheng1, Jun-Yu Chen2, Bing Chen3
1School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350002, PR China.
Clinical oral investigations
|February 19, 2025
概括
从二氧化玻璃陶 (LDGC) 表面上完全去除Monobond Etch & Prime (MEP) 残留物是不可能实现的. 然而,牙医可以通过延长冲洗时间和使用超声波清洁来提高MEP清洁的效率.
科学领域:
- 牙科材料科学 牙科材料科学
- 分析化学 分析化学
- 生物材料是一种生物材料.
背景情况:
- 莫诺邦 Etch & Prime (MEP) 用于粘合陶修复.
- 脱酸玻璃陶 (LDGC) 表面上的残留MEP可能会影响临床结果.
- 需要一种敏感的方法来量化清洗后的MEP残留物.
研究的目的:
- 开发一种高度敏感和可靠的HPLC-TTQ/MS方法来检测MEP残留物.
- 评估不同清洁程序对从LDGC表面去除MEP残留物的有效性.
主要方法:
- 开发并验证了一种HPLC-TTQ/MS方法,用于测量四甲二三化物 (TDTF).
- 用MEP处理了100个LDGC标本.
- 在不同的冲洗时间 (3-9s),牙医经验和超声波清洗 (UC) 后评估了TDTF残留物.
主要成果:
- 通过HPLC-TTQ/MS方法证明了高线性 (r2 > 0.999) 和灵敏度 (LOQ < 5 ng/mL).
- 在所有组中检测到残留的TDTF,范围从62.42 ng到74,611.74 ng.
- 经验丰富的牙医,更长的冲洗时间 (9s) 和UC显著减少了TDTF残留物 (P <0.05).
- UC特别有效,在所有疾病中显著降低了TDTF水平 (P ≤0.012).
结论:
- 没有经过测试的清洁方案可以完全消除LDGC的MEP残留物.
- 临床经验,冲洗时间和超声波清洗影响MEP残留物水平.
- 超声波清洗是最大限度地减少MEP残留物的高效补充.
相关概念视频
01:11High-Performance Liquid Chromatography: Introduction
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
01:05High-Performance Liquid Chromatography: Elution Process
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...