相关实验视频

Updated: May 27, 2025

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

在Saccharomyces属中探索适应低温的适应路径

Javier Pinto¹, Laura Natalia Balarezo-Cisneros¹, Daniela Delneri¹

¹Faculty of Biology Medicine and Health, Manchester Institute of Biotechnology, The University of Manchester, Manchester, United Kingdom.

|February 19, 2025

概括

研究Saccharomyces酵母的寒冷适应性揭示了转录调节,而不仅仅是基因变异,对于在不断变化的环境中生存至关重要. 这突显了微生物进化的复杂遗传基础.

科学领域:

微生物遗传学和适应能力
进化生物学是进化的生物学.
酵母研究的酵母研究.

背景情况:

了解微生物适应温度等环境因素对生物多样性至关重要.
具有多样化的耐热性Saccharomyces属作为研究适应性等位基因的模型.
以前的研究已经确定了参与寒冷适应的基因.

研究的目的:

为了对所有八种Saccharomyces物种中参与寒冷适应的六个候选基因进行全属评估.
为了研究S. kudriavzevii和S. eubayanus.寒冷耐受性的进化路径.
区分基因表达和等位基因变异在寒冷适应中的作用.

主要方法:

在八种Saccharomyces物种中对六个候选基因的全属评估.
在自然分离物中对基因表达的分析,特别关注氧化还原平衡基因.
试验操作涉及促进体和编码序列 (CDS) 替换两个基因在四种酵母菌种.

主要成果:

耐寒性在S. kudriavzevii和S. eubayanus中通过不同的途径进化,涉及氧化还原平衡和脂肪酸代谢基因.
观察到温度和物种依赖的表皮病,表明复杂的遗传相互作用.
与S. cerevisiae相比,S. kudriavzevii,S. jurei和S. mikatae的自然分离物中氧化还原平衡基因的表达更高.
从S. kudriavzevii进行促销者交换显著改善了寒冷适应性,而CDS交换导致了较低的增长.

结论:

由促进体活动驱动的转录强度,在寒冷适应中比编码序列发挥更重要的作用.
在Saccharomyces中适应寒冷的温度很可能以基因表达网络的随机调整为中心.
这项研究强调了微生物适应过程中遗传相互作用的可塑性和复杂性.

更多相关视频

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Published on: September 17, 2016

相关概念视频

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Overview of Secretory Vesicles

Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.