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Updated: May 27, 2025

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使用C-标记的内部标准来推进甲酸的分析

Zhangjie Wang1, Jian Liu2

  • 1Glycan Therapeutics Corp., Raleigh, North Carolina, United States.

American journal of physiology. Cell physiology
|February 19, 2025
PubMed
概括

这项研究引入了一种先进的LC-MS/MS方法,使用同位素标记的标准来精确量化甲酸盐 (HS). 这种技术可以对生物系统中的HS修饰和代谢进行详细的分析.

关键词:
有13C标签的内部标准.HS二糖化/3-O-硫酸四糖化HS代谢的代谢方式HS代谢方式这是LC-MS/MS.硫酸盐酸酶的使用方法

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Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
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