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Jing Chen¹, Wei Zhang², Yang Li¹

¹Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.

|February 19, 2025

概括

一种新的蝙蝠冠状病毒,HKU5-CoV 2系,有效地使用人类的 ACE2,这表明存在潜在的动物感染风险. 这种病毒表现出明显的结合性和广泛的宿主热带性,与其他merbecoviruses不同.

科学领域:

病毒学
结构生物学
动物传染病研究

背景情况:

梅尔贝科病毒表现出遗传多样性,但动物菌株对人类的溢出风险未得到充分研究.
蝙蝠冠状病毒HKU5 (Pterac-CoV HKU5) 是已知的四种merbecovirus之一.

研究的目的:

调查蝙蝠病毒对人类的潜在传播风险.
以其受体使用和宿主热带性来描述新发现的HKU5-CoV系2 (HKU5-CoV-2).

主要方法:

用冷电子显微镜 (Cryo-EM) 分析HKU5-CoV-2受体结合域 (RBD) 的复合体与人类血管素转化酶2 (ACE2).
进行结构和功能分析,将HKU5-CoV-2与其他merbecoviruses进行比较,并评估其适应人类ACE2.
使用人类ACE2表达细胞系和人类呼吸道和肠道器官的感染研究.

主要成果:

在蝙蝠中发现HKU5-CoV系2 (HKU5-CoV-2),它有效地利用人类的ACE2作为功能性受体.
冷EM揭示了HKU5-CoV-2 RBD与人类ACE2的独特结合方式,与其他merbecoviruses不同.
与1型HKU5-CoV相比,HKU5-CoV表现出更广泛的宿主热带性和更好的适应人类ACE2.
真正的HKU5-CoV-2成功感染了人类的ACE2表达细胞和人体器官.

结论:

HKU5-CoV-2 代表了一种独特的蝙蝠merbecoviruses,具有显著的动物传播潜力.
病毒有效利用人类的ACE2和广泛的宿主热带性突出显示了其潜在的公共健康威胁.

关键词:

一个蝙蝠低温电磁分析主体热带性人类ACE2 血统 2 HKU5 艾滋病病毒病毒隔离

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