含有nisin的凝微粒用于增强细菌纳米纤维素的生物活性
Maša Hren1, Janja Trček2, Aleksandra Šakanović3
1Faculty of Mechanical Engineering, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia.
International journal of biological macromolecules
|February 19, 2025
概括
用凝和尼修饰的细菌纳米纤维素显示出增强的抗菌性质. 这种修改改善了细胞活力,并为生物医学应用提供了有前途的方法.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 抗菌研究 抗菌研究
背景情况:
- 细菌纳米纤维素 (BnC) 是医学中的重要生物材料,但缺乏固有的生物活性.
- 修改BnC对于提高其治疗潜力和克服局限性至关重要.
研究的目的:
- 开发一种使用凝和尼修饰细菌纳米纤维素 (BnC) 的方法.
- 研究这些生物分子对BnC抗菌活性和生物相容性的协同作用.
主要方法:
- 通过喷雾干燥和脱水热处理制备带有或没有尼载荷的凝微粒.
- 用凝微粒和/或尼修改BnC膜.
- 使用人类牙纤维细胞 (HGF) 和尿道细胞评估细胞活力.
- 对抗黄金葡萄球菌 (Staphylococcus aureus) 的抗菌活性的评估.
主要成果:
- 修改后的BnC膜支持矿物质的形成,对HGF细胞无细胞毒性.
- 仅仅是凝微粒就提高了HGF的生存能力~20%.
- 与尼/凝溶液不同的是,尼涂层的凝微粒保持了高细胞活力.
- BnC 膜在尿细胞中显示>90%的活力.
- 经过修改的膜在非细胞毒性尼剂量下对金黄色葡萄球菌表现出抗菌活性.
结论:
- 凝和尼的修改显著影响BnC的生物活性和抗菌疗效.
- 尼辛涂层的凝微粒提供了一种安全有效的方法,可以为BnC赋予抗菌性质.
- 这种方法为开发医疗用途的先进生物活性BnC基材料提供了可行的策略.
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