在食道状细胞癌中,FBXL18增加细胞增殖并降低细胞辐射敏感性
Yahui Kang1,2, Ning Ge1,2, Xiaolong Yuan1,2
1Department of Radiation Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, 230031, Hefei, Anhui, China.
概括
富含F盒和白的重复蛋白18 (FBXL18) 通过增加细胞增殖和降低放射敏感性,促进食道状细胞癌 (ESCC) 的进展. 这种瘤效应是由FBXL7在ESCC细胞中的下调调节的介导.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 食道状细胞癌 (ESCC) 是一种普遍存在的全球性恶性瘤.
- 了解驱动ESCC进展的分子机制对于开发有效疗法至关重要.
研究的目的:
- 调查F-box和富含白的重复蛋白18 (FBXL18) 在ESCC病变发生过程中的作用.
- 阐明FBXL18影响ESCC细胞行为和辐射敏感性的机制.
主要方法:
- 在ESCC细胞系中使用lentiviral载体,FBXL18过度表达或被击倒.
- 细胞活力,增殖和辐射敏感性使用细胞计数工具-8和殖民地形成等测试来评估.
- 瘤生长在体内通过向裸体小鼠注射改性ESCC细胞来评估.
- 蛋白质表达的变化通过西方 blot. 分析.
主要成果:
- 过度表达FBXL18显著提高了ESCC细胞的活力,增殖和殖民地形成.
- 在体内,FBXL18 knockdown 抑制了瘤生长.
- 过度表达FBXL18降低了ESCC细胞的辐射敏感性.
- 从机理上讲,发现FBXL18抑制了FBXL7.7的表达.
结论:
- FBXL18在ESCC中充当瘤蛋白,促进细胞增殖和降低辐射敏感性.
- FBXL18的亲瘤效应可能通过FBXL7.7的下调调节来调节.
- 针对FBXL18可能是ESCC的治疗策略.
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