Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

6.2K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
6.2K
Non-gated Ion Channels01:24

Non-gated Ion Channels

6.6K
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
6.6K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

2.1K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.1K
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

7.9K
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several...
7.9K
Drug Elimination by Renal Route: Tubular Secretion01:15

Drug Elimination by Renal Route: Tubular Secretion

2.1K
Once the process of glomerular filtration is completed, blood carrying unfiltered drug molecules traverses through efferent arterioles and makes its way into the peritubular capillaries in the proximal tubule. A variety of carriers play a pivotal role in actively secreting drugs from these peritubular capillaries into the tubular fluid. The organic anion transporter transfers acidic drugs, against an electrochemical gradient, from the peritubular capillaries into the renal tubule cells and...
2.1K
Pore Transport and Ion-Pair Transport01:17

Pore Transport and Ion-Pair Transport

332
Pore transport and ion-pair formation are critical mechanisms for the absorption and distribution of drugs in the body.
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited  but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct...
332

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

In Vitro Characterization of Cannabidiol As a Possible Adjuvant for Long-Lasting Local Anesthesia.

Anesthesia and analgesia·2026
Same author

Defining AV2-1 as a novel pharmacological probe to target human and rodent TRPV2.

British journal of pharmacology·2026
Same author

Molecular mechanism of menthol-induced TRPV5 channel inhibition.

Nature communications·2026
Same author

The Excelsatoxin A-Receptor TMEM233 Modulates Nav1.8.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Structures of nucleotide-bound Redondovirus Rep protein link conformation and function.

PLoS pathogens·2026
Same author

UVA Light Triggers Activation of TRPV1 and TRPA1 by Staurosporine and Midostaurin.

International journal of molecular sciences·2026
Same journal

Publisher Correction: Interplay between cohesin and RNA polymerase II in regulating chromatin interactions and gene transcription.

Nature structural & molecular biology·2026
Same journal

An asymmetric non-canonical nucleosome shapes the directionality of transcription outcomes.

Nature structural & molecular biology·2026
Same journal

Structural insights into neurokinin 2 receptor selectivity hold implications for obesity therapeutics.

Nature structural & molecular biology·2026
Same journal

Genome-wide absolute quantification of chromatin looping.

Nature structural & molecular biology·2026
Same journal

Putting numbers on chromatin looping.

Nature structural & molecular biology·2026
Same journal

Transcriptional readthrough progresses from incidental byproduct to therapeutic opportunity.

Nature structural & molecular biology·2026
查看所有相关文章

相关实验视频

Updated: May 27, 2025

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

10.1K

通过probenecid对TRPV2调制的结构见解.

Julia A Rocereta1,2, Toni Sturhahn3, Ruth A Pumroy1,2

  • 1Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Nature structural & molecular biology
|February 19, 2025
PubMed
概括
此摘要是机器生成的。

普罗贝尼西德 (PBC) 通过结合新型细胞内口袋来强化TRPV2通道,稳定活性构造. 这一发现通过准TRPV2功能,为心血管疾病提供了新的治疗策略.

更多相关视频

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3
07:26

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Published on: August 17, 2011

21.1K
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

7.9K

相关实验视频

Last Updated: May 27, 2025

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

10.1K
Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3
07:26

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Published on: August 17, 2011

21.1K
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

7.9K

科学领域:

  • 离子通道研究
  • 心血管生理学心血管生理学
  • 药理学 药理学是指药理学的学科.

背景情况:

  • 暂时受体潜在化物2 (TRPV2) 通道对心血管健康和疾病至关重要.
  • 一种尿素酸剂Pobenecid (PBC) 已显示出改善心血管功能的潜力.

研究的目的:

  • 为了研究Pobenecid (PBC) 与TRPV2离子通道相互作用并调节其活性的机制.
  • 阐明TRPV2.2的PBC增强的结构基础.

主要方法:

  • 电生理学被用来测量TRPV2通道活性.
  • 低温电子显微镜提供了与TRPV2.2结合的PBC的高分辨率结构数据.
  • 用定位突变发生法来研究特定氨基酸的作用.

主要成果:

  • 普罗贝尼西德 (PBC) 显著增强了老鼠的TRPV2活性.
  • 冷EM揭示了TRPV2上PBC的新型细胞内结合口袋,其中涉及一个保存的histidine残留物.
  • PBC结合防止了通道不活化,并且可以通过胺替代扩展到TRPV1和TRPV3通道.
  • 与2-aminoethoxydiphenyl borate结合的PBC会诱导一个增强的TRPV2状态.

结论:

  • 普罗贝尼西德 (PBC) 在一个独特的细胞内口袋中直接与TRPV2结合,稳定了潜在的通道构造.
  • 这种相互作用机制为TRPV2调节提供了结构基础,并表明心血管疾病的治疗潜力.