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通过全面的转录组学和蛋白组学分析,识别与绵羊单边密码学相关的关键基因

Sheng-Wei Pei¹, Yang-Kai Liu¹, Zhong-Yu Wang¹

¹State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.

|February 19, 2025

概括

雄羊的密码症对繁殖和绵羊产业产生了重大影响. 这项研究确定了密虫丸中改变的关键基因和通路,为精子生成失败提供了洞察力.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
蛋白质组学是指蛋白质组学.

背景情况:

密码症是一种常见的公羊生殖缺陷,导致绵羊行业的重大经济损失.
有限的研究存在于绵羊中密码的遗传基础上.

研究的目的:

为了研究雄羊中单边密码化背后的分子机制.
为了识别cryptorchid丸中差异表达的基因和蛋白质.

主要方法:

使用RNA-seq. 的转录组分析.
使用基于TMT的蛋白质组学进行蛋白质组分析.
生物信息分析包括功能丰富和Venn图.

主要成果:

在正常和形丸之间确定了数千个差异表达基因 (DEG).
发现了与编程细胞死亡,新陈代谢和精子生成相关的基因的显著变化.
结合的转录基因和蛋白质基因数据显示了325个重叠的基因,确定了10个候选关键基因.

结论:

基因和蛋白质的表达在单边鱼公羊的丸中发生了显著的变化.
确定了潜在的候选基因和信号通路,涉及单边密码学.
提供了新的洞察力,了解了密码化中精子生成失败的分子基础.

关键词:

密码主义是什么?密码主义是什么?生育能力生育能力生育能力绵羊是一种羊.精子发生是精子生成.测试 (Testis) 是一个测试.

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