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Updated: May 27, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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从逆相色谱到HILIC:图形变压器电源方法独立机器学习的保留时间

Cailum M K Stienstra1, Emir Nazdrajić1, W Scott Hopkins1,2,3

  • 1Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

Analytical chemistry
|February 20, 2025
PubMed
概括

图形转换器 (Graphormer-RT) 是一种新的图形转换器,用于方法独立地预测液态染色学 (LC) 保留时间 (RT). 这种方法使得在质谱学 (MS2) 中更准确的代谢物注释和标识成为可能.

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Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
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