连续高通量等离子分离用于血液生物标志物传感,使用水力动力微流体装置.
Hesam Abouali1, Fatemeh Keyvani1, Seied Ali Hosseini1
1Department of Electrical and Computer Engineering, University of Waterloo, Waterloo, ON, N2L 3G1, Canada.
Advanced healthcare materials
|February 20, 2025
概括
一个新的微流体装置提供连续的,高通量血分离. 这种液态连续,高通量等离子分离器 (HCHPS) 实现了高纯度和生物标志物检测的产量.
科学领域:
- 生物医学工程 生物医学工程
- 流体动力学 流体动力学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 有效的血分离对于临床生物标志物检测至关重要.
- 现有的离心和微流体等现有方法在吞吐量,成本,产量和纯度方面存在局限性.
- 需要连续,高通量等离子体分离技术,以满足临床需求.
研究的目的:
- 开发和验证一种微流体装置,用于连续,经济高效,高通量血分离.
- 优化微通道设计,以提高等离子体提取产量和纯度.
- 确认分离血适合下游生物标志物分析.
主要方法:
- 使用被动水力学原理设计和制造微流体装置.
- 计算和实验评估,以优化用于等离子提取的侧通道长度.
- 用全血和稀释血液测试设备,评估纯度,产量和血液溶解.
- 使用基于珠的光和电化学的阿帕特马生物传感器验证血质量.
主要成果:
- 开发的水力动力连续高通量血分离器 (HCHPS) 装置达到47-64%的纯度和10-18%的全血产量,与离心法相比降低了血液溶解.
- 分离稀释的血液产生了62-97%的纯度与相似的产量.
- 使用通过光和电化学生物传感分离的血进行成功的下游生物标记分析.
结论:
- HCHPS微流体装置为连续,高通量等离子体分离提供了有效的解决方案.
- 优化的设计确保了允许的产量和纯度,适合临床生物标志物检测.
- 这项技术为准备血样本的传统方法提供了一个有希望的替代方案.
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