翻译后的裂变在蛇中产生截断的IgY形式Elaphe taeniura
Ming Zhang1, Long Hua2, Tang-Yuan Xie2
1Key Laboratory of Qinghai-Xizang Plateau Animal Genetic Resource Reservation and Utilization, Sichuan Province and Ministry of Education, Southwest Minzu University, Chengdu, Sichuan 610041, China.
Zoological research
|February 20, 2025
概括
免疫球蛋白常数区域通过各种机制多样化. 这项研究揭示了一种新的翻译后裂,在蛇中产生截断的IgY,与其他脊椎动物不同.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 免疫球蛋白 (Ig) 常数区域通过诸如类切换重组 (CSR) 和子类差异化等机制进行多样化.
- 在鸟类,爬行动物和两动物中存在截断的IgY异型 (缺乏Fc域),这些异型是由转录或遗传差异引起的.
- 之前的IgY(ΔFc) 变体与本研究中发现的新型截断形式不同.
研究的目的:
- 为了研究产生蛇*Elaphe taeniura*中截断的IgY异型的机制.
- 描述这种截断的IgY的结构和分子基础.
- 将这种机制与其他脊椎动物已知的IgY多样化策略进行比较.
主要方法:
- 西方涂抹用于检测和描述蛋白质碎片.
- 液体染色学-并联质谱学 (LC-MS/MS) 用于精确识别裂变点.
- 在实验室中使用蛇和人类阿斯巴拉基尼尔内酶进行裂解分析.
主要成果:
- 在 *Elaphe taeniura* 蛇中发现了一种新的截断IgY异型,仅缺少CH4域的一部分.
- 在IgY重链常数区域内的阿斯巴拉金残留N338的翻译后裂解被证实是产生机制.
- 蛇和人类的阿斯巴拉基尼尔内酶都在试验室中证明了分裂蛇IgY的能力.
结论:
- 免疫球蛋白CH区域表现出各种策略,用于在脊椎动物物种中产生功能变异.
- 翻译后的酶分离代表了一种新发现的产生缩短IgY异型的机制.
- 这一发现扩大了我们对爬行动物IgY多样化和进化的理解.
相关概念视频
Protein Folding Quality Check in the RER
3.7K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
3.7K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Post-translational Translocation of Proteins to the RER
5.6K
A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
5.6K
Insertion of Single-pass Transmembrane Proteins in the RER
6.5K
Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
6.5K
Transcytosis of IgG
2.7K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
2.7K
Protein and Protein Structure
77.9K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
A protein's shape is critical to its function. For example, an enzyme...
77.9K


