相关实验视频
Updated: May 27, 2025

10:43
Dissection of Larval Zebrafish Gonadal Tissue
Published on: April 26, 2017
10.2K
在银唇珍珠 (Pinctada maxima) 中,基于整合性转录基因分析解读性别差异化的遗传基础
Zi-Jian Li1, Zhi-Hui Yang1, Jia-Hui Wang1
1Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya, Hainan 572000, China.
Zoological research
|February 20, 2025
概括
研究人员在银唇珍珠 (Pinctada maxima) 中确定了控制性别决定和差异化的关键基因. 这种分子理解可以改善珍珠的繁殖,以获得更好的珍珠质量和产量.
科学领域:
- 海洋生物学 海洋生物学
- 基因组学就是基因组学.
- 水产养殖是水产养殖的一种方式.
背景情况:
- 银唇珍珠 (Pinctada maxima) 在商业上对珍珠生产至关重要.
- 珍珠的两性变异需要了解繁殖的性别决定机制.
- 目前关于P.maxima分子性决定的知识有限.
研究的目的:
- 阐明Pinctada maxima中性别决定和分化的分子机制.
- 确定参与卵巢和丸发育的关键基因和途径.
- 为改善珍珠繁殖策略提供分子基础.
主要方法:
- 综合性转录组分析使用异形测序 (Iso-seq) 和RNA测序 (RNA-seq).
- 卵巢和丸淋巴细胞组织的比较分析.
- 基因共同表达网络分析和替代拼接分析.
主要成果:
- 在卵巢和丸组织之间鉴定了2,768个差异表达基因 (DEG).
- 发现了关键的性别决定/维护基因 (如FOXL2,DMRT) 和性别特异的共同表达模块.
- 揭示了结合体途径和替代拼接在生殖腺发育中的重要作用,特别是在丸中.
结论:
- 在P.maxima中证实了与性别相关的基因的保存,突出了它们在卵巢和丸维护中的作用.
- 在P.maxima性腺体中展示了广泛的替代拼接和差异转录使用.
- 这些发现为有针对性的繁殖策略提供了分子基础,以控制性别和增强珍珠生产.
相关概念视频
The Ratio of X Chromosome to Autosomes
8.4K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
8.4K
Background and Environment Affect Phenotype
6.4K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.4K
Dosage Compensation
6.1K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.1K
Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Genetic Screens
4.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
4.8K
Pleiotropy
39.5K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
39.5K

