电可以通过调节宿主衍生的miR-205-5p来缓解功能性便秘,以调节肠道微生物群和酸盐代谢
Lu Wang1,2, Menghan Xi2,3, Wei Cao4
1Department of Acupuncture, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
电针 (EA) 疗法可提高 miR-205-5p 的调节,通过促进有益的细菌,如 Lactobacillus reuteri,增强肠道运动性. 这项研究确定了一种治疗功能性便秘 (FC) 的新机制.
科学领域:
- 胃肠道学和分子生物学
- 微生物组研究 微生物组研究
- 针和神经调节治疗
背景情况:
- 功能性便秘 (FC) 是一种普遍存在的胃肠道疾病.
- 电针 (EA) 通过改善肠道运动性,显示了对FC的治疗潜力.
- 微RNAs (miRNAs) 是宿主微生物群相互作用的关键调节者,但它们在EA治疗便秘中的作用尚不清楚.
研究的目的:
- 确定EA治疗便秘小鼠中的便miRNA及其参与微生物群调节和肠道运动的标.
- 阐明EA影响肠道微生物组和宿主生理学的机制.
- 研究特定微生物代谢物的治疗潜力,以缓解便秘.
主要方法:
- 在小鼠中用洛佩拉胺诱导的便秘模型,用电针 (EA) 治疗.
- 对便微生物组合和结肠miRNA表达的分析.
- 在体内对肠道微生物群 (抗生素) 和miRNA标 (AAV) 进行机械验证.
主要成果:
- 在接受EA治疗的小鼠的便和结肠中,miR-205-5p被显著上调.
- EA治疗通过miR-205-5p增加了Lactobacillus reuteri的丰度,改善了肠道运动性.
- 乳杆菌reuteri及其托芬代谢物 (英多尔衍生物) 改善了洛佩拉胺诱导的便秘.
结论:
- 来自宿主的miR-205-5p在通过微生物组调节来增强肠道运动性方面发挥着至关重要的作用.
- EA疗法促进有益细菌及其代谢物,为功能性便秘提供了一种新的治疗策略.
- miR-205-5p/Lactobacillus reuteri轴代表了一个关键途径,将EA治疗与改善肠道功能联系起来.
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