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相关概念视频

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

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Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
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Lysosomal Hydrolases01:22

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Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
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Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

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The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
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相关实验视频

Updated: May 27, 2025

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
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线粒体功能障碍驱动自然杀手细胞功能障碍在系统性红斑狼.

Natalia W Fluder, Morgane Humbel, Emeline Recazens

    medRxiv : the preprint server for health sciences
    |February 20, 2025
    PubMed
    概括

    系统性红斑狼 (SLE) 损害了自然杀手 (NK) 细胞,因为缺陷线粒体细胞吸食导致线粒体功能障碍. 用乌罗立A恢复线粒可以改善SLE患者的NK细胞功能.

    科学领域:

    • 免疫学 免疫学 免疫学
    • 细胞生物学 细胞生物学
    • 线粒体生物学 线粒体生物学

    背景情况:

    • 系统性红斑狼 (SLE) 是一种由免疫失调和炎症标志着的自身免疫性疾病.
    • 自然杀手 (NK) 细胞对于免疫监测至关重要,但在SLE中表现出功能受损,包括细胞毒性降低和细胞因子产生.
    • 在SLE中NK细胞功能障碍背后的精确机制,特别是关于线粒体健康的机制,尚未完全理解.

    研究的目的:

    • 为了研究功能障碍的线粒体和受损的线粒体在SLENK细胞损伤中的作用.
    • 探索潜在的治疗策略,通过向SLE中线粒体质量控制来恢复NK细胞功能.

    主要方法:

    • 评估了104名SLE患者和104名健康对照者的NK细胞中的线粒体结构和功能,使用流细胞计,传输电子显微镜和蛋白质组学.
    • 通过RT-qPCR.量化了与mitophagy相关的基因表达.
    • 评估了Urolithin A (线粒细胞活化剂) 和氧化 (HCQ) 对线粒体循环和NK细胞功能in vitro的影响.

    主要成果:

    • SLE NK 细胞显示扩大,功能障碍的线粒体,受损的溶酶体酸性化,以及细胞质线粒体DNA泄漏的增加,表明线粒体缺陷.
    • 蛋白质组和转录组分析揭示了SLENK细胞中与线粒细胞相关的基因的下调,与减少的效应因子功能 (降粒,细胞因子产生) 相对应.

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  • 在体外用乌罗立A治疗增强了线粒,改善了线粒体和溶酶体功能,并恢复了NK细胞效应因子的反应;HCQ显示部分恢复.

    • 结论:

    • 线粒体功能障碍和线粒体衰竭是SLENK细胞异常的关键因素.
    • 这项研究揭示了一种新的免疫代谢机制,它是SLE病原体的基础.
    • 准线粒体质量控制途径为SLE提供了一个有希望的治疗途径.