通过Bacillus velezensis的计算流体动力学模拟,高效地生产聚-γ-胺酸,用于制造冷面团面包
Hong Liu1, Qiaojuan Yan1, Yuchuan Wang2
1Key Laboratory of Food Bioengineering (China National Light Industry), College of Engineering, China Agricultural University, Beijing 100083, China.
Food chemistry: X
|February 20, 2025
概括
百菌 velezensis CAU263使用料批发发酵产生高产的聚-γ-胺酸 (γ-PGA). 优化的螺旋设计显著提高了γ-PGA的生产,并证明了其作为烤中的冷保护剂的潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
背景情况:
- 聚-γ-胺酸 (γ-PGA) 是一种多功能生物聚合物,具有众多应用.
- 优化微生物发酵过程对于提高生物聚合物产量至关重要.
研究的目的:
- 为了评估和增强由 *Bacillus velezensis* CAU263.3 的聚-γ-胺酸 (γ-PGA) 生产.
- 通过计算流体动力学 (CFD) 研究推动器优化对g-PGA产量的影响.
- 评估γ-PGA作为冷保护剂在冷面团应用中的有效性.
主要方法:
- 在5L发酵器中*Bacillus velezensis* CAU263的料批发发酵.
- 计算流体动力学 (CFD) 模拟以优化螺旋配置.
- 对γ-PGA收益率和转换率的分析.
- 评估冷面团的特性 (特定体积,硬度) 用g-PGA添加.
主要成果:
- 实现了初始的 γ-PGA 产量为 60.4 g/L,转化率为 0.97 g/g.
- 优化的螺旋组合 (六个半圆形叶片的Rushton轮机和四个曲的宽叶片螺旋) 增加了g-PGA产量,达到80.7g/L (1.29g/g转换率).
- 添加 γ-PGA 改善了冷面团,导致特定体积增加了 21.3%,硬度降低了 38.3%.
结论:
- 通过料批发发酵和基于CFD的推进器优化,开发了一种提高B. velezensis* γ-PGA生产的有效策略.
- 该研究表明,γ-PGA作为制行业的冷保护剂具有显著的潜力,可以提高冷面团产品的质量.
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