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在体外基因编辑使用从表达Cas9的猪中获得的原始细胞

Seokho Kim¹, Jin-Gu No¹, Seunghoon Lee¹

¹Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.

Journal of animal science and technology

|February 20, 2025

概括

研究人员开发了一种新的Cas9表达猪模型,用于高效的基因编辑. 这个平台简化了为农业和生物医学进步创造转基因猪的流程.

科学领域:

遗传学是一个遗传学.
动物生物技术动物生物技术
分子生物学分子生物学

背景情况:

聚类正规间隔短时间的Palindromic重复/CRISPR相关蛋白9 (CRISPR/Cas9) 技术使猪的基因编辑成为可能.
产生基因工程猪目前是时间和劳动密集型.
由于Cas9核酶的大小,现有的体内基因编辑方法面临效率挑战.

研究的目的:

开发一个更有效的平台来产生基因编辑猪.
为简化基因编辑创建具有构成Cas9表达的猪模型.
为体内和体外基因编辑应用建立一个资源.

主要方法:

通过将 Cas9 基因集成到 ROSA26 位点中,生成了一个转基因猪.
在产生的猪的各种组织中确认了构成性Cas9表达.
评估了表达Cas9的猪的生育能力.
使用来自猪的原始细胞进行了体外基因编辑实验.

主要成果:

成功生成了一个具有构成性Cas9表达的肥沃猪.
在试验室中通过将单一指导RNA (sgRNA) 添加到原始细胞中来证明有效的基因删除.
ROSA26位点促进了稳定和广泛的Cas9表达.

结论:

开发的表达Cas9的猪可以作为基因编辑的有效平台.
这种模式显著简化和加速了转基因猪的生产.
该平台有潜力通过高效的基因编辑推进农业和生物医学研究.

关键词:

这就是Cas9的情况.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪 sgRNARNA 是一个

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