优化和半连续发酵的葡萄糖-寡糖的生产与微菌YRK005 cibaria
Sungyoon Kim1, Jisun Park1, Huijin Jeong1
1Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 Republic of Korea.
Food science and biotechnology
|February 20, 2025
概括
使用Weissella cibaria优化葡萄糖-寡糖的生产需要半连续发酵. 有氧半连续发酵显著提高了生产效率,提供了一个有前途的工业战略.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 发酵科学 发酵科学
背景情况:
- 葡萄糖-寡糖 (GOS) 是具有显著健康益处的益生菌.
- 对工业应用而言,高效和成本效益的GOS生产至关重要.
- 韦塞拉西巴利亚是GOS生产的潜在微生物来源.
研究的目的:
- 为了优化使用Weissella cibaria YRK005.5.的葡萄糖-寡糖酸盐 (GOS) 生产.
- 为了确定影响GOS产量的关键发酵参数.
- 评估半连续发酵策略,以提高GOS生产.
主要方法:
- 普莱克特-伯曼设计用于识别关键因素 (糖,麦芽糖,温度).
- 响应表面方法 (RSM) 与中央复合设计用于参数优化.
- 无氧和有氧批量发酵的比较分析.
- 优化有氧半连续发酵与介质补充.
主要成果:
- 确定了最佳条件:14.7%的糖,13.5%的麦芽糖,30°C,预计相对峰值面积 (RPA) 为115.6.6.
- 有氧批量发酵 (25.5 RPA/h) 的生产率高于无氧发酵 (23.7 RPA/h).
- 有氧半连续发酵在一个循环中实现了39.2 RPA/h,优于两循环 (27.2 RPA/h).
结论:
- 发酵条件对于增强葡萄糖-寡糖酸盐的产生至关重要.
- 有氧半连续发酵是一种高效的策略,可以提高GOS产量.
- 这种优化的工艺显示出对具有成本效益的工业GOS生产的前景.
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