相关实验视频

Updated: May 27, 2025

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
05:33

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

9.4K

蛋白质体抑制增强了溶酶体介导的向蛋白质降解

Ahmed M Elshazly, Nayyerehalsadat Hosseini, Shanwei Shen

    bioRxiv : the preprint server for biology
    |February 20, 2025
    PubMed
    概括

    癌细胞在对蛋白酶体抑制剂的反应中激活自适应性自. 这项研究利用这种反应,使用向自的嵌合体 (AUTAC) 来降解Mcl1,增强蛋白酶体抑制剂的有效性并促进癌细胞死亡.

    更多相关视频

    Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae09:05

    Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

    Published on: April 18, 2016

    28.8K
    Assaying Proteasomal Degradation in a Cell-free System in Plants07:43

    Assaying Proteasomal Degradation in a Cell-free System in Plants

    Published on: March 26, 2014

    14.4K

    相关概念视频

    The Proteasome01:13

    The Proteasome

    Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
    In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
    801
    Regulated Protein Degradation02:58

    Regulated Protein Degradation

    It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
    Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
    7.1K
    The Proteasome Structure01:17

    The Proteasome Structure

    The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
    The proteasome is an...
    677
    Export of Misfolded Proteins out of the ER01:32

    Export of Misfolded Proteins out of the ER

    After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
    3.4K
    Lysosomal Hydrolases01:22

    Lysosomal Hydrolases

    Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
    3.7K
    Delivery Pathways to the Lysosome01:36

    Delivery Pathways to the Lysosome

    Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
    Endocytosis
    In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
    6.1K