在单个染色体驱动单质型内对抗性基因素-14s
Meghan J Brady1, Anjali Gupta2, Jonathan I Gent3
1Department of Genetics, University of Georgia, Athens Georgia 30602, USA.
bioRxiv : the preprint server for biology
|February 20, 2025
概括
玉米中性驱动系统 (异常染色体10和K10L2) 中的TRKIN基因似乎对驱动效率和健康有负面影响,这表明它可能被从种群中清除.
科学领域:
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 玉米的介质驱动系统,异常染色体10 (Ab10) 和K10L2,运行在串联重复阵列 (旋) 上.
- Ab10编码的是KINDR/knob180和TRKIN/TR-1中华素;K10L2只编码的是TRKIN/TR-1.
- 之前的研究表明,KINDR/knob180是主要的驱动因素,TRKIN/TR-1则起到较小的作用.
研究的目的:
- 调查TRKIN/TR-1系统在玉米中介驱动中的作用.
- 使用长读序列测序来表征K10L2单元型.
- 评估TRKIN基因功能对Ab10和K10L2介质驱动力和健身的影响.
主要方法:
- 长阅读测序组装K10L2单元型.
- 通过CRISPR的突变发生来使Trkin基因失活.
- 建模以预测特尔金的人口动态.
主要成果:
- K10L2与Ab10.的内部区域具有同质性.
- 当与正常的染色体10结合在一起时,Trkin on Ab10对驱动和健身有轻微的有害影响.
- 在Ab10或K10L2上的功能Trkin在与K10L2.2配对时,严重减少了Ab10驱动器.
结论:
- 特尔金基因不会增强Ab10的驱动力或健身,可能有害.
- 在Ab10种群中Trkin的持久性是可疑的.
- 特尔金可能会带来未被观察到的优势,或者正在从A10人群中清除.
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