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剪切应激调节促进了猪内心内皮细胞的亲炎性反应

Pengfei Ji^1,2,3,4,5, Kathryn Jane Grande-Allen⁶, Swathi Balaji^1,4,5

¹Laboratory for Regenerative Tissue Repair, Texas Children's Hospital, Houston, Texas, USA.

bioRxiv : the preprint server for biology

|February 20, 2025

概括

离散性静脉下狭窄症 (DSS) 与内心内皮细胞 (EECs) 对剪切应激的反应有关. 这项研究发现,当EEC暴露于剪切应力时,会释放调解DSS的炎症性细胞因子.

科学领域:

心血管生物学心血管生物学
细胞机械传导细胞机械传导
遗传性心脏病研究遗传性心脏病研究

背景情况:

离散性静脉下狭窄 (DSS) 是一种先天性心脏缺陷,影响左心室外流通道 (LVOT).
内心内皮细胞 (EECs) 参与了DSS,但它们对LVOT中剪切应激的反应尚不清楚.

研究的目的:

调查EEC对剪切应力的反应在调解DSS中的作用.
测试EECs对LVOT剪压的反应是DSS开发的关键因素的假设.

主要方法:

猪EEC在形和板式生物反应器中受到受控的剪切应力.
在条件介质中测量了益炎性细胞因子度,使用Luminex测定.
大量RNA测序分析了剪切应力EEC中的基因型变化,包括CD31表达.

主要成果:

剪切应力诱导了EEC中PI3K-AKT和TNF-a信号通路的激活.
EECs释放了精选的促炎细胞因子,并触发了免疫细胞分化通路 (Th1,Th2,Th17).
鉴定出CD31是剪切压力EECs中促炎反应的调解者.

结论:

切割应力和EECs的炎症反应之间确立了直接联系.
这种EECs的炎症反应在DSS的发病过程中起到调解作用.

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