相关实验视频

Updated: Apr 24, 2026

High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection

High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection

Published on: December 18, 2017

获得安普利康序列标志着一个自私的老鼠 - - 哈普类型

Callie M Swanepoel¹, Gaojianyong Wang², Lucy Zhang¹

¹Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI, USA.

bioRxiv : the preprint server for biology

|February 20, 2025

概括

室内小鼠中的自私基因基因带动大基因组区域的偏向遗传. 阿姆普利康和t-哈普类型的反转在300万年前进化,塑造了17号染色体的进化.

科学领域:

遗传学遗传学是一个
进化生物学进化生物学
基因组学就是基因组学.

背景情况:

门德尔遗传学假定等等基因传播,但自私的遗传因素可以破坏这一点.
室内小鼠的t-半型表现出极端的传播偏差,与第17号染色体的大量反转有关.
这种大规模传播偏差的进化起源仍然不太清楚.

研究的目的:

调查自私等位基因的遗传结构和进化史,以驱动Mus musculus t-haplotypes中的传播偏差.
确定对自私染色体17的进化负责的基因组特征.

主要方法:

生成了一个t-haplotype的高质量的单个哈普类型组件.
分析了amplicon结构,基因含量和表达模式.
进行了有针对性的删除实验,以评估候选自私等位基因的功能.

主要成果:

揭示了八个大安普利康作为t-haplotypes的独特特征,含有已知和候选自私等位基因.
在相关的Mus物种中确定了保存的amplicons,这表明自私元素的早期起源 (~3 MYA) 与反转相关.
发现富含精子,逆原体和差异表达基因表达的基因.
证明删除一个约1.8Mb的安普利康可以减少3%的传输偏差.

结论:

安普利康收购和大反转是自私染色体17的起源和演变的关键驱动因素.
自私的等位基因含有安普利康的进化与哺乳动物的性染色体进化相似.
自私的单元类型参与进化军备竞赛,用片和反向作为自私传播的基因组签名.

更多相关视频

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

相关概念视频

DNA-only Transposons

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...

Horizontal Gene Transfer

Horizontal Gene Transfer

Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...