相关实验视频
Updated: May 27, 2025

15:42
Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
Published on: March 6, 2009
21.7K
CD4粘膜关联不变T (MAIT) 细胞表达高度多样化的T细胞受体
bioRxiv : the preprint server for biology
|February 20, 2025
概括
具有CD4共同表达的粘膜关联不变T细胞 (MAIT细胞) 使用不同的T细胞受体 (TCR),与正规的CD8+MAIT细胞不同. 这表明替代MR1配体可能会影响CD4+MAIT细胞的选择.
科学领域:
- 免疫学 免疫学 免疫学
- T细胞生物学T细胞生物学
- 这是一种与生俱来的类似免疫力.
背景情况:
- 粘膜关联不变T细胞 (MAIT) 是保存的与生俱来的类似T细胞,在免疫中起着关键的作用.
- 规范MAIT细胞表达CD8αβ+和T细胞受体 (TCR) 的TRAV1-2+,识别由MR1.1呈现的微生物代谢物.
- 新兴证据表明,各种MAIT细胞子集,包括CD4+群体,具有潜在的独特功能.
研究的目的:
- 为了研究人类CD4+MAIT细胞中T细胞受体 (TCR) 谱的异质性.
- 用一种不偏见的方法来描述CD4+MAIT细胞的TCR序列.
- 探索CD4+和CD8+MAIT细胞子集之间TCR使用的潜在差异.
主要方法:
- 在MR1-5-OP-RU-tetramer-reactive T细胞上采用了无偏的单细胞TCR测序方法.
- 在两个独立的数据集中,分析了单个MR1-5-OP-RU四相酶阳性MAIT细胞的VDJ序列.
- 在体外扩展后检查的TCR剧目发生了变化,包括与Mycobacterium tuberculosis.
主要成果:
- 发现CD4+MAIT细胞表达多种TCR是TRAV1-2负的,与正规的MAIT细胞不同.
- 在 CD4+ MAIT 细胞中确定了独特的 TCR 使用,包括 TRAV21, TRAV8 和 TRAV12 家族,具有可变的 J 链和 CDR3 序列.
- 在体外扩张时观察到非TRAV1-2 MAIT细胞TCR的丰富,表明它们的功能相关性.
结论:
- 成熟的人类CD4+ MAIT细胞与正规的CD8+ TRAV1-2+ MAIT细胞子集相比,表现出明显的TCR使用.
- CD4+ MAIT 细胞中多样化的 TCR 列表表明,除了 riboflavin 中间体之外,还可以通过替代 MR1 配体进行选择.
- 这一发现扩大了我们对MAIT细胞异质性及其在免疫反应中的潜在作用的理解.
更多相关视频
08:48Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
Published on: October 25, 2016
8.4K
08:11Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
Published on: September 1, 2015
8.7K
相关概念视频
Diversity of Antigen Receptors
490
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
490
T Cell Activation and Clonal Selection
629
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
629
Antigens Involved in Adaptive Immunity
438
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
Complete Antigens
Complete antigens possess both immunogenicity and...
438
Special Features of Adaptive Immunity
728
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
728
T Cell Types and Functions
897
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
897