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Updated: May 27, 2025

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
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乳标签使得在细胞培养中能够进行蛋白质组广泛的循环动力学分析

Lorena Alamillo1,2, Dominic C M Ng1,2, Jordan Currie1,2

  • 1Department of Medicine, University of Colorado School of Medicine, Aurora, CO 80045, USA.

bioRxiv : the preprint server for biology
|February 20, 2025
PubMed
概括

这项研究揭示了人类诱导多能干细胞 (hiPSC) 中的动态蛋白质循环,确定了100多种新的快速循环蛋白质,并展示了差异化如何改变蛋白质降解模式.

关键词:
蛋白质的营业额降级 degron 降级 降低 降低沉水是重水,重水是重水,重水是重水.这是 hiPSC.质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量蛋白质合成 蛋白质合成一些秘密一些秘密.营业额的动力学

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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