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优化了用于Aureobasidium pullulans的基因工程的载体

Analeigha Colarusso¹, Audrey Williams², Amy S Gladfelter²

¹Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.

bioRxiv : the preprint server for biology

|February 20, 2025

概括

研究人员开发了新的遗传工具来研究Aureobasidium pullulans,一种多功能黑酵母. 这种扩展的工具包有助于在各种环境中研究细胞适应和形状调节.

科学领域:

菌类学菌类学是指菌类学.
合成生物学合成生物学
分子生物学分子生物学

背景情况:

Aureobasidium pullulans是一种多极耐性黑酵母,以其形态可塑性而闻名.
它的适应性使它成为研究细胞适应和形状调节的有希望的模型生物.

研究的目的:

为了扩大Aureobasidium pullulans的遗传工具包.
在A. pullulans中创建用于基因表达,标记和删除的多功能载体.

主要方法:

设计和测试25种新型载体.
包括七个编码子优化的光体 (例如,GFP,mCherry).
开发用于双表达的载体,基于同质的基因删除和不进行克隆的C端标记.

主要成果:

一系列用于A. pullulans研究的25个功能载体成功创建和验证.
载体使双蛋白表达,内源基因标记和基因删除成为可能.
新的工具支持URA3位点的实验,并促进基于同质性的修改.

结论:

开发的矢量序列显著提高了研究Aureobasidium pullulans的实验能力.
这种多功能工具包将加速在这个新兴模型系统中对细胞适应和形态发生的研究.

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