生物相容的基于硫的共价探针用于活细胞和固定细胞中的内源性管氨酸光纳米镜
Marie Auvray1, Tanja Koenen2, Olexandr Dybkov3
1Chromatin Labeling and Imaging group, Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077 Göttingen, Germany.
bioRxiv : the preprint server for biology
|February 20, 2025
概括
研究人员开发了一种新的共价光探针,6-SiR-CTX,用于精确的管成像. 这种探针具有高细胞透性和最小的管功能干扰,使高级超分辨率显微镜成为可能.
科学领域:
- 细胞生物学 细胞生物学
- 分子成像学分子成像学
- 生物化学 生物化学
背景情况:
- 光探头和超分辨率显微镜对于可视化细胞动态至关重要.
- 现有的方法,如光蛋白融合或联结光体的光体可能会导致功能障碍.
- 图布林是一种关键的细胞骨蛋白质,需要精确的成像来理解细胞过程.
研究的目的:
- 开发一种对内源性管氨酸的共振光探针.
- 为了克服传统光标签方法的局限性.
- 为了能够在最小的细胞干扰下高分辨率地成像管动力学.
主要方法:
- 基于cabazitaxel和-rhodamine的新型共价光探针6-SiR-CTX的设计和合成.
- 加入一个生物相容的可切割连接器与一个硫中心.
- 在各种人类细胞系中使用活细胞和固定细胞成像,包括STED纳米镜,评估探针性能.
主要成果:
- 6-SiR-CTX探针显示出出色的细胞透性和原性质.
- 在多个人类细胞系中实现了内源性突素的共价标记.
- 准部分是可移除的,保留了管的染色和功能,与STED纳米镜相容.
结论:
- 新型共价光探针6-SiR-CTX为高分辨率的管成像提供了一个强大的工具.
- 这种方法最大限度地减少了管功能的干扰,比传统技术提供了优势.
- 探测器与STED纳米镜的兼容性增强了它在研究动态细胞过程中的实用性.
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