基因组RNA依赖和独立机制调节B淋巴细胞中的X染色体动态无活化
bioRxiv : the preprint server for biology
|February 20, 2025
概括
雌性B细胞中的X染色体失活 (XCI) 表观遗传记忆依赖于XistRNA. 不活跃的X染色体 (Xi) 上的特定基因组标记在原始细胞中建立,并在刺激后保持.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 哺乳动物遗传学 哺乳动物遗传学
- 细胞生物学 细胞生物学
背景情况:
- 在X染色体无活化 (XCI) 中,X染色体同等于X相关基因表达.
- 雌性B细胞中的XCI动态显示了动态表观遗传痕迹.
- 基质RNA和异色标记在原始B细胞中不存在,但在刺激时会重新出现.
研究的目的:
- 研究B细胞中不活跃X染色体 (Xi) 上的异色素组标记.
- 确定XistRNA是否需要在刺激后沉积和保留这些标记.
主要方法:
- 在Xi.上分析基因组修饰 (H2AK119Ub,H3K9me3,H3K27me3) 和DNA甲基化.
- 评估XistRNA在表观遗传标记维护中的作用.
- 对B细胞中毒性刺激模型的研究.
主要成果:
- 纯粹的B细胞Xi缺少H2AK119Ub和H3K9me3,但对DNA甲基化和H3K27me3.3进行丰富.
- 这些标记建立了XCI的XistRNA依赖的表观遗传记忆.
- 刺激导致Xist独立的H3K27me3和Xist依赖的H2AK119Ub在Xi上的积累.
结论:
- 在刺激的女性B细胞中,XistRNA,H3K27me3和H2AK119Ub对于保持X链基因表观遗传完整性至关重要.
- XCI的表观遗传记忆在原始B细胞中建立,并在激活时动态调节.
更多相关视频
12:42Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
Published on: November 26, 2014
13.9K
08:27A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
Published on: May 22, 2019
6.3K
相关概念视频
X-Inactivation
38.0K
The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
38.0K
Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
Dosage Compensation
6.1K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.1K
Chromatin Structure Regulates pre-mRNA Processing
6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
6.9K
Heterochromatin
9.8K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
9.8K
B Cell Activation and Differentiation
1.5K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.5K
