基于Levon开发纳米颗粒的研究,用于口服胰岛素输送
Desy Kurniawati1, Neng Fisheri Kurniati2, Enny Ratnaningsih1
1Biochemistry Research Division, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Bandung 40132, Indonesia.
Biomedical materials (Bristol, England)
|February 20, 2025
概括
这项研究开发了乙化Levon纳米颗粒 (I-ALv) 用于口服胰岛素的输送. I-ALv显示增强了胰岛素稳定性,并在体内有效降低了血糖水平.
科学领域:
- 生物材料科学 生物材料科学
- 药物运输 药物运输 药物运输
- 纳米技术 纳米技术
背景情况:
- 口服胰岛素因胃酸降解而面临挑战.
- 生物聚合物纳米载体为保护胰岛素提供了一个潜在的解决方案.
- 莱万 (Levan) 是一种来自 *Bacillus licheniformis * 的生物聚合物,研究了胰岛素封装.
研究的目的:
- 评估Levane及其乙化形式作为口服胰岛素输送的纳米载体.
- 为了评估胰岛素的稳定性和释放特征,该胰岛素被封装在基于激素的纳米颗粒中.
- 研究开发的纳米载体系统用于糖尿病管理的体内疗效.
主要方法:
- 莱万被提取并乙化以产生莱万 (I-Lv) 和乙化莱万 (I-ALv) 纳米粒子 (NP).
- 在模拟的胃液中确定了胰岛素封装效率和释放动力学.
- 在NP中分析了胰岛素的结构稳定性,使用循环二极化和光光谱学.
- 在体内研究评估了I-ALv对血糖水平的影响.
主要成果:
- 产生了具有高封装效率的球形NP (250-500nm) (78.64%的ILV,88.30%的I-ALV).
- 与I-Lv NPs相比,I-ALv NPs在模拟的胃液中表现出更慢的胰岛素释放率.
- 在I-ALvNP中的胰岛素显著增强了二级和三级结构稳定性.
- 在体内初步研究表明,I-ALv有效降低了血糖水平.
结论:
- 乙化Levon纳米颗粒 (I-ALv) 是一个有前途的系统,可以提供口服胰岛素.
- I-ALv增强了胰岛素的结构稳定性,保护它免受降解.
- 这种纳米载体系统有可能成为有效的抗糖尿病疗法.
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