相关实验视频
Updated: Aug 1, 2026
07:18
Microinjection of Zebrafish Embryos to Analyze Gene Function
Published on: March 9, 2009
77.4K
表皮转录因子grhl3的丧失导致斑马鱼的发育表型具有可变的透性
Nishanthi Mathiyalagan1, Travis K Johnson2, Zachary Di Pastena1
1Department of Microbiology, Anatomy, Physiology and Pharmacology (MAPP), La Trobe University, Bundoora, Victoria, Australia.
概括
环境因素显著影响斑马鱼的发育,影响缺乏粒头样3 (grhl3) 基因的胚胎. 这项研究揭示了环境变异如何影响GRHL3-nullizygous胚胎中的基因表达和发育结果.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 皮质生物学 皮质生物学
背景情况:
- 环境影响对胚胎发生至关重要,但在实验室环境中经常被忽视.
- 转录因子 Grainyhead-like 3 (grhl3) 对于上皮屏障的形成,神经和面发育至关重要.
- 实验室条件可能无法充分捕捉环境变异对发育过程的影响.
研究的目的:
- 研究对缺乏grhl3基因的斑马鱼发育表型的环境影响.
- 在发育生物学背景下了解基因环境相互作用.
- 探索ghrhl3在上皮质平衡中的作用.
主要方法:
- 使用具有grhl3基因删除的斑马鱼模型 (grhl3-无菌胚胎).
- 在不同的水生设施环境中比较grhl3-/-胚胎的表型表现.
- 观察和描述发育现象,包括身体长度,面发育和肠道屏障维护.
主要成果:
- 之前报告的11小时后GRHL3-/-胚胎的胚胎死亡率 (hpf) 被环境条件改变.
- 在不同的水生设施中,Grhl3-/-胚胎表现出三种不同的表型:体长缩短,耳面和头骨面异常以及肠道屏障受损.
- 环境条件导致部分表型透到基因相同的grhl3-/-胚胎中.
结论:
- 这项研究建立了一个新的模型,用于研究在基因相同的胚胎中部分表型透.
- 这种模型对于理解发育中的基因环境相互作用和上皮质平衡是有价值的.
- 环境变化在发育过程和遗传特征表达中起着重要的,被低估的作用.
相关概念视频
01:33Pleiotropy
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
02:27Background and Environment Affect Phenotype
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...