雌激素防止了由 Porphyromonas gingivalis 脂多糖酸盐引起的牙屏障损伤
Fangting Huang1,2, Zhifei Su1,3, Fangjie Zhou1,3
1State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
Infection and immunity
|February 20, 2025
概括
雌激素,特别是β-雌激醇 (E2),通过加强牙上皮屏障来保护牙周病. 这涉及增加紧结蛋白,减少炎症,并防止由Porphyromonas gingivalis引起的骨损失.
科学领域:
- 口腔生物学 口腔生物学
- 内分泌学 在内分泌学.
- 免疫学 免疫学 免疫学
背景情况:
- 绝经后的女性由于雌激素水平下降而经历更严重的牙周病.
- 雌激素在膜骨再吸收中发挥作用,这是牙周炎的关键特征.
- 牙上皮质屏障的完整性对于预防病原体引起的炎症性损伤至关重要.
研究的目的:
- 调查雌激素对牙上皮质屏障对 Porphyromonas gingivalis 诱导的炎症的保护作用.
- 为了确定雌激素能否保持屏障完整性,并减少人类牙上皮细胞和小鼠模型中的炎症反应.
主要方法:
- 在人类牙上皮细胞 (hGECs) 和卵巢切除/沙姆小鼠中使用了Porphyromonas gingivalis脂多糖化物 (LPS) 的感染模型.
- 采用qPCR,西部涂抹,免疫组织化学和转录组分析来评估屏障功能和蛋白质表达.
- 评估了雌激素对紧结蛋白,炎症和膜骨再吸收的影响.
主要成果:
- 雌激素显著恢复了上皮屏障功能,抑制了P. gingivalis-LPS的入侵,并减少了炎症 (P <0.05).
- 雌激素在hGEC和小鼠的mRNA和蛋白质水平上调节了紧结蛋白 (JAM1,OCLN) 的表达 (P <0.05).
- 雌激素在卵巢切除和Sham小鼠 (P <0.05) 中表现出对膜骨再吸收的保护作用,并保持了屏障完整性.
结论:
- β-雌二醇 (E2) 有效地防止了由P. gingivalis-LPS引起的牙上皮质屏障损伤和炎症的进展.
- 雌激素通过增加关键紧结蛋白的表达来增强屏障完整性.
- 雌激素在维护牙屏障功能的保护作用表明,在牙周炎和相关的炎症性疾病中具有潜在的治疗应用.
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