基因引入策略使得能够构建一个多产的真菌二次代谢物库
Aoi Kimishima1,2, Sota Honma1,2, Satoshi Kato2
1O̅mura Satoshi Memorial Institute, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Journal of natural products
|February 20, 2025
概括
将LaeA基因引入真菌中可以提高二次代谢产量. 这项研究成功创建了一个真菌菌株库,产生了超过十倍的自然产品增加,如radicicol和sch210972.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物技术是生物技术.
- 化学生物学 化学生物学
背景情况:
- LaeA是一种影响二次代谢产生的真菌蛋白.
- 之前关于LaeA功效的研究仅限于少数真菌物种.
- 对于改善真菌菌株的LaeA的广泛适用性在很大程度上仍未被探索.
研究的目的:
- 研究LaeA基因在多种真菌属中修改二次代谢物产生的实用性和多功能性.
- 通过向以前未经修改的物种引入LaeA基因来建立真菌菌株库.
- 分析生成的突变体的化学生产力和生物活性.
主要方法:
- 从三个属 (Pochonia,Gamszarea,Lecanicillium) 中选择了47种以前未经LaeA.修改的真菌菌株.
- 通过将LaeA基因引入选定的真菌菌株,产生125个突变.
- 分析由此产生的真菌菌株库的化学生产率和生物活性.
主要成果:
- 通过使用LaeA基因进行工程设计的125种真菌突变的图书馆已成功创建.
- 分析显示,化学生产率和生物活性发生了显著变化.
- 两种自然产品的分离,radicicol (1) 和sch210972 (2),产量超过母菌株的十倍.
结论:
- LaeA基因是提高各种真菌物种中二次代谢物产生的多功能工具.
- 使用LaeA的工程真菌菌株可以显著增加有价值的自然产品的产量.
- 这种方法对发现和生产由真菌衍生的新型化合物具有前景.
更多相关视频
相关概念视频
00:57Antibiotic Selection
Overview
01:24Evolution of New Traits in Microbes
Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...
01:22Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
01:27Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
01:18Production of Biopesticides
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
01:24iChip
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...