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在新辅助疗法后的瘤区域的全面MALDI质谱成像

Hui Xu¹, Qian Zhang^1,2, Jie Yuan²

¹School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in University of Shandong, Yantai University, Yantai, 264005, P. R. China.

Analytical and bioanalytical chemistry

|February 20, 2025

概括

这项研究优化了矩阵辅助激光分解/电离质谱成像 (MALDI MSI) 用于在新辅助化疗 (NAC) 后分析脆弱的瘤组织. 新方法保留了死角区域,揭示了化学疗法诱导的关键空间代谢变化.

科学领域:

在瘤学瘤学.
代谢学代谢学代谢学
生物化学生化学

背景情况:

在新辅助化疗 (NAC) 后对瘤组织的空间代谢分析对于理解治疗效果至关重要.
矩阵辅助激光消化/电离量谱成像 (MALDI MSI) 是高分辨率组织代谢学的关键技术.
在NAC后组织中,脆弱的死角区域对样本准备和准确的MALDI MSI分析构成重大挑战.

研究的目的:

开发和验证一个优化的工作流程,用于马尔迪MSI分析后NAC瘤组织.
在样本准备和分析过程中克服与脆弱的死组织区域相关的挑战.
为了使在NAC后,在活跃和死瘤区域的代谢变化的详细空间映射.

主要方法:

开发一种优化的样品准备工作流程,使用导电带进行组织稳定.
在NAC后瘤组织的切割和MALDI MSI分析中应用优化的工作流程.
对同一组织样本的死细胞区域和活细胞区域的代谢概况进行比较分析.

主要成果:

导电带方法成功地保持了组织完整性,特别是在脆弱的死角区域.
在NAC后瘤组织的不同区域成功地绘制了空间解析的代谢资料.
在NAC后,在死和可活瘤区域中发现了明显的代谢变化,提供了新的见解.

结论:

使用导电带的优化MALDI MSI工作流对分析具有挑战性的NAC后瘤组织非常有效.
这种技术增强了MALDI MSI在瘤学研究中的空间解析代谢学的能力.
这些发现为化学疗法诱导的瘤内代谢重编程提供了宝贵的见解.

关键词:

矩阵辅助激光消耗离子化质谱仪.尸体死后的区域是一个死后的区域.新辅助化学疗法新辅助化学疗法空间代谢学空间代谢学

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