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Jing Yang¹, Liming Liu², Junhui Li¹

¹School of Life Science, Henan Institute of Science and Technology, Hualan Road, Xinxiang, Henan, 453000, China.

Archives of virology

|February 20, 2025

概括

在患病的植物中发现了一种新型的花蜜相关的cytorhabdovirus (HaCV). 遗传学分析显示,一个分离物体代表了一种新物种,而另外四种属于Triticum alphacytorhabdovirus 1.

科学领域:

植物病毒学植物病毒学
分子生物学分子生物学
人类遗传学是一个学科.

背景情况:

细胞哈勃病毒是具有细分RNA基因组的感染植物的病毒.
观察到蜜植物表现出化和斑点症状.
高通量测序是新型病毒发现的强大工具.

研究的目的:

为了识别和表征一种可谓的新型蜂病毒在蜂.
为了确定新型病毒与已知的cytorhabdoviruses的遗传关系.
根据基因组序列的身份对已识别的病毒分离物进行分类.

主要方法:

具有症状的蜜植物的高通量测序.
基因组组装和对开放式阅读框架 (ORF) 的分析.
遗传学分析和全基因组序列比较.

主要成果:

鉴定了五种cytorhabdovirus分离物,暂时命名为花蜜相关的cytorhabdovirus (HaCV).
基因组结构分析揭示了八个ORF,包括正规和额外的假定蛋白质 (P", P3, P6).
遗传学分析显示,分离物最接近Triticum alphacytorhabdovirus 1 (TriACRV1);然而,FQ3分离物显示<75%的序列相同性,符合新物种的标准,而FQ1,FQ2,FQ4和FQ5分离物显示>75%的同样性与TriACRV1.

结论:

隔离FQ3代表了Cytorhabdovirus属内的新物种.
隔离物FQ1,FQ2,FQ4和FQ5被归类为TriACRV1隔离物.
这项研究扩大了已知的cytorhabdoviruses及其宿主的多样性.

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