Search research articles

相关概念视频

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.

Nature methods·2026

Same author

A critical initialization for biological neural networks.

Nature·2026

Same author

TFIIIC regulates SMC complex binding and 3D DNA contacts between tRNA genes.

bioRxiv : the preprint server for biology·2026

Same author

A series of spontaneously blinking dyes for super-resolution microscopy.

Nature methods·2026

Same author

Midgestation metabolic constraint in purine metabolism drives distinct strategies for placenta and fetal growth.

bioRxiv : the preprint server for biology·2026

Same author

Innovative In Vivo Imaging and Single Cell Expression from Tumor Bulk and Corpus Callosum Reveal Glioma Stem Cells with Unique Regulatory Programs.

Cancers·2025

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

Search research articles

相关实验视频

Updated: May 4, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

高空间分辨率的深层组织转录和亚细胞成像

Valentina Gandin¹, Jun Kim¹, Liang-Zhong Yang¹

¹Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

Science (New York, N.Y.)

|February 20, 2025

概括

新的循环杂交连锁反应 (cycleHCR) 成像克服了有限的光通道,用于生物样本中RNA和蛋白质的详细空间分析,从而使深层组织研究成为可能.

更多相关视频

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Author Spotlight: Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue

Author Spotlight: Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue

Published on: September 8, 2023

相关实验视频

Last Updated: May 4, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Author Spotlight: Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue

Author Spotlight: Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue

Published on: September 8, 2023

科学领域:

分子生物学
显微镜技术
基因组学

背景情况:

传统的光显微镜受限于可用的颜色通道数量,限制了多个目标的同时空间分析.
在复杂的生物系统中分析RNA和蛋白质的空间组织需要先进的成像方法.

研究的目的:

引入和验证循环杂交链反应 (循环HCR) 作为一种高度多重化的RNA和蛋白质成像的新方法.
展示循环HCR在整个胚胎和亚细胞结构中的3D空间分析的能力.

主要方法:

通过将多循环DNA条码与杂交连锁反应 (HCR) 结合而开发的循环HCR.
将循环HCR应用于整个胚胎转录组图像,并将其与扩展显微镜相结合,以获得亚细胞分辨率.
在小鼠海马片中利用循环HCR进行多重RNA和蛋白质成像.

主要成果:

在整个胚胎中实现精确的3D基因表达和细胞命运映射 (~310微米深).
使用循环HCR和扩展显微镜在小鼠胚胎纤维细胞中揭示了复杂的亚细胞结构 (10种).
在小鼠海马片中发现复杂的基因表达梯度和细胞类型特定的核变异.

结论:

循环HCR提供了一个多功能和定量框架,用于阐明深层组织的空间调节.
这种方法克服了传统光显微镜用于多重成像的局限性.
对于生物研究和诊断应用而言, cycleHCR具有显著的潜力.