相关实验视频

Updated: May 27, 2025

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

在进化指导下发现了类似于拉索的抗菌菌酸

Aske Merrild¹, Tiziana Svenningsen¹, Marc G Chevrette^2,3

¹Department of Biological & Chemical Engineering, Aarhus University, Gustav Wieds vej 10D, 8000, Aarhus, Denmark.

Angewandte Chemie (International ed. in English)

|February 20, 2025

概括

三胺是一种具有抗菌素活性的拉索,通过一种独特的途径产生. 它的乙化是一种抗性机制,在多个细菌族群中发现了类似的,表明了广泛的分布和进化.

科学领域:

微生物学微生物学
生物化学生物化学
分子生物学分子生物学

背景情况:

三胺是一种核糖体合成和翻译后修饰的 (RiPP) 拉索.
它表现出强大的抗菌细菌活性,并由非正规生物合成基因集群 (BGC) 生产.

研究的目的:

为了阐明三胺的生物合成途径.
调查乙化在三胺生物合成和活性中的作用.
探索三胺和相关的拉索的分布和演变.

主要方法:

三胺BGC的异质表达.
在体外乙化和生物活性测定.
基因组搜索和BGCs的遗传学分析.

主要成果:

生物合成需要前体 (triA),宏循环酶 (triC) 和N-乙转移酶 (triT).
通过triT的乙化赋予了电阻.
在至少三个细菌系中发现了类似三胺的,这表明分布比以前知道的更广泛.
发现了新的拉索,棕胺和凝胺,而凝胺具有额外的环状结构.
遗传学分析表明,三胺类宏环酶的演变趋同.

结论:

这项研究揭示了三胺的生物合成途径和抵抗机制.
三胺和相关的拉索呈现出显著的进化多样化和跨系分布.
这些发现扩大了RiPP拉索演变和功能的已知景观.

关键词:

抗生素的发现抗生素的发现融合进化的趋同.不同类的表达式不同类的表达式拉索酸是一种拉索酸.这就是RiPPs.

更多相关视频

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

Published on: November 2, 2016

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...