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基于细胞位置评估多细胞系统中细胞的机械特征

Hiroshi Koyama¹, Atsushi M Ito², Hisashi Okumura³

¹Division of Embryology, National Institute for Basic Biology, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan; SOKENDAI (The Graduate University for Advanced Studies), Hayama, Kanagawa 240-0193, Japan.

Journal of theoretical biology

|February 20, 2025

概括

这项研究将亚细胞力与细胞-细胞相互作用力联系起来. 细胞-细胞粘附是有吸引力的,而表面张力和细胞-ECM粘附是排斥性的,解释了多细胞形态.

科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
计算生物学计算生物学

背景情况:

了解多细胞生物的形态需要测量细胞-细胞相互作用的力量.
之前的方法使用粗粒度模型推断了双向力,但缺乏亚细胞细节.

研究的目的:

为了研究有效的双向细胞-细胞力量和亚细胞机械元件之间的关系.
为了提供一个理论框架,将推断的力量与潜在的物理性质联系起来.

主要方法:

将基于图像的统计推理方法应用于来自顶点模型的细胞跟踪数据.
分析了各种亚细胞组件 (粘附,张力,ECM相互作用,生长) 对有效力的贡献.

主要成果:

确定的细胞-细胞粘附力具有吸引力.
发现细胞表面张力和细胞-细胞外矩阵 (ECM) 粘附力是排斥性的.
已证明的亚细胞成分对有效的细胞-细胞相互作用力有显著的贡献.

结论:

亚细胞机械性质直接影响新出现的细胞-细胞相互作用力.
这项工作可以从细胞位置数据中预测亚细胞组件.
在发育生物学中解释基于粒子的模拟结果的基础.

关键词:

细胞细胞相互作用有效潜在的有效潜力.基于图像的力推断推断.机械学机械学机械学粒子模型中的粒子模型.垂直模型的模型

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