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阿斯普罗辛和格雷姆林-1表达在埃及1型糖尿病儿科患者中的预测作用
Al-Shimaa M Abas1, Marwa M Esawy2, Fatma Kamel3
1Biochemistry Department, Faculty of Science, Zagazig University, PO Box- 44519, Zagazig, Egypt. dr_shmma@yahoo.com.
Scientific reports
|February 20, 2025
概括
在1型糖尿病患者 (T1DM) 中,阿斯素和格雷姆林-1基因表达和蛋白质水平显著升高. 阿斯素表达显示T1DM诊断的高准确性,表明其作为诊断标记物的潜力.
科学领域:
- 内分泌学 在内分泌学.
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
背景情况:
- 1型糖尿病 (T1DM) 涉及胰腺β细胞的自身免疫破坏,导致胰岛素缺乏.
- 亚斯素和格雷姆林-1在T1DM病变发生过程中的确切作用仍然不完全理解.
- 研究新生物标志物对于早期T1DM检测和管理至关重要.
研究的目的:
- 与健康对照组相比,评估T1DM患者中阿斯素和格雷姆林-1的表达水平.
- 确定阿斯普罗辛和格雷姆林-1作为T1DM生物标志物的诊断潜力.
- 评估阿斯普罗辛和格雷姆林-1表达与发展T1DM的风险之间的关联.
主要方法:
- 从T1DM患者和年龄/性别匹配的对照组收集了血清样本和周围血液.
- 通过实时PCR量化阿斯普罗辛和格雷姆林-1的基因表达水平.
- 通过ELISA测量了阿斯素和格雷姆林-1的血清蛋白度.
主要成果:
- 在T1DM患者中,Asprosin和Gremlin-1基因表达和血清蛋白水平都显著更高 (P < 0.001).
- 阿斯素表达表明T1DM的高诊断准确性,具有96%的灵敏度,92%的特异性和94%的准确性.
- 阿斯素表达与T1DM风险增加276倍有关,而格雷姆林-1表达与风险增加84.33倍有关.
结论:
- 阿斯素和格雷姆林-1在T1DM患者中显著上调,这表明它们与疾病有关.
- 阿斯素基因表达成为T1DM诊断的高度准确和有前途的生物标志物.
- 升高的阿斯素和格林林-1表达与患T1DM的风险增加密切相关.
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