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相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: May 27, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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使用SEC-seq将单细胞转录组与分泌物联系起来.

Justin Langerman1, Sevana Baghdasarian2, Rene Yu-Hong Cheng3,4

  • 1Department of Biological Chemistry, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, USA.

Nature protocols
|February 20, 2025
PubMed
概括
此摘要是机器生成的。

科学家们开发了SEC-seq,这是一种新方法,将蛋白质分泌水平与单细胞中的基因表达联系起来. 这一突破有助于了解细胞功能,并通过分析分泌的生物分子来设计更好的细胞疗法.

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Last Updated: May 27, 2025

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 细胞分泌出各种生物分子,用于细胞通信和病原体防御等关键功能.
  • 细胞疗法的疗效依赖于特定分子的分泌,如细胞因子和生长因子.
  • 了解决定细胞分泌模式的基因表达状态至关重要,但很大程度上是未知的.

研究的目的:

  • 开发一种新的协议,将分泌的标蛋白质数量与单细胞中的转录序列连接起来.
  • 为了能够更深入地了解基因表达和分泌表现型之间的关系.
  • 为了促进改进的基于细胞的疗法的工程.

主要方法:

  • 开发了SEC-seq (通过测序与基因表达相关的分泌量) 使用微尺度的水凝颗粒 (纳米粒子).
  • 纳米细胞捕获细胞及其分泌物,使用带有寡核酸标记的抗体标记分泌物.
  • 综合流细胞计和单细胞RNA测序 (scRNA-seq) 用于同时分析细胞转录组和分泌水平.

主要成果:

  • 成功将T细胞受体序列与细胞因子分泌水平联系起来.
  • 根据表面标记物基因表达,确定了一种高度分泌的,潜在的再生性细胞群.
  • 在血细胞中具有高免疫球蛋白分泌率的相关转录组.

结论:

  • SEC-seq提供了一种多功能方法来量化蛋白质分泌,并将其与各种细胞类型的基因表达联系起来.
  • 该协议很快,关键步骤在不到4小时内完成.
  • SEC-seq为研究细胞分泌提供了可通用的解决方案,进步了我们对细胞疗法的理解和工程.