Trps1通过PDE4D/AKT/CREB信号通路调节小鼠胚胎基因组激活和植入前胚胎发育
Xia Jiang1, Weiwei Xu1, Jiandong Sun1,2,3,4
1Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
Cell biology and toxicology
|February 20, 2025
概括
母因子TRPS1对于小鼠早期发育至关重要,通过PDE4D/AKT/CREB通路调节胚胎基因组激活 (ZGA). 它的缺失会影响胚胎发育,突出显示TRPS1的存在.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
背景情况:
- 紫毒基因组激活 (ZGA) 对哺乳动物胚胎发育至关重要.
- 管理ZGA的准确监管机制在很大程度上是未知的.
研究的目的:
- 研究母因子TRPS1在小鼠植入前胚胎发育中的作用.
- 阐明TRPS1介导的ZGA调节中涉及的分子通路.
主要方法:
- 在小鼠胚胎中利用siRNA介导的Trps1敲除.
- 研究了Trps1对ZGA和植入前发育的影响.
- 分析了AKT/CREB信号通路和Pde4d基因表达.
- 通过Pde4d mRNA联合注射进行了救援实验.
主要成果:
- 缺少Trps1会影响ZGA和从2细胞胚胎过渡到4细胞胚胎.
- Trps1 缺乏导致AKT/CREB信号通路的失调.
- TRPS1调节Pde4d转录,影响AKT和CREB的酸化.
- 同时注射Trps1siRNA和Pde4dmRNA挽救了发育缺陷.
结论:
- TRPS1是对小鼠植入前发育的关键母因子.
- TRPS1通过准PDE4D/AKT/CREB通路来调节ZGA.
- TRPS1在小鼠早期胚胎发生过程中起着负面的调节作用.
相关概念视频
01:22PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K
01:33Cleavage and Blastulation
44.6K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
44.6K