相关实验视频

Updated: May 27, 2025

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

CDCA3-MYC正反循环通过ENO1-介导的糖解促进了膀癌的进展

Dexin Shen¹, Xiang Yu², Xuefeng Fan²

¹Department of Urology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, China. mltyyds@ustc.edu.cn.

Journal of experimental & clinical cancer research : CR

|February 20, 2025

概括

细胞循环调节器CDCA3通过与MYC形成反循环,促进膀癌 (BLCA) 糖解和进展. 这一发现为BLCA病原体和潜在的治疗点提供了新的见解.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

膀癌 (BLCA) 诊断依赖于侵入性方法,目前的化疗有局限性.
需要新的生物标志物和策略来提高BLCA诊断和治疗疗效.
CDCA3与BLCA的进展有关,但其调节机制需要进一步阐明.

研究的目的:

研究CDCA3在膀癌 (BLCA) 进展中的功能作用.
阐明CDCA3调节BLCA的分子机制.
为了确定预测BLCA患者存活率的潜在生物标志物.

主要方法:

用MTT,殖民地形成和流动细胞计量来评估细胞增殖和细胞亡.
基因表达和蛋白质相互作用通过qRT-PCR,西部抹杀和Co-IP测试进行了分析.
使用CDCA3和ENO1表达数据开发了一个风险预测模型.

主要成果:

CDCA3通过调节α-enolase (ENO1) 来促进BLCA糖解,从而推动瘤的进展.
通过TRIM28调节的CDCA3和MYC之间的正反循环被确定,稳定MYC并调节CDCA3.
基于CDCA3和ENO1表达的预后模型有效预测BLCA患者的整体存活率.

结论:

在调节BLCA糖解和进展方面,CDCA3发挥着新的作用.
CDCA3-MYC反循环是BLCA病变发生的一个关键机制.
CDCA3和ENO1表达水平可以作为膀癌的宝贵预后生物标志物.

关键词:

膀癌是一种癌症.这是一个CDCA3CDCA3CDCA3CDCA3CDCA3.葡萄糖溶解是什么? 葡萄糖溶解我的世界 MYC 在 TRIM2828 中,

更多相关视频

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids

Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids

Published on: October 21, 2022

相关概念视频

Adaptive Mechanisms in Cancer Cells

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...