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普奇尼亚状菌效应器PNPi抑制TaIAA14的表达以抑制宿主细胞死亡反应

Huiyutang Wang¹, Xue Gao¹, Yixi Kong¹

¹College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.

Molecular plant pathology

|February 21, 2025

概括

普西尼亚形效应剂PNPi通过向辅酶信号来抑制植物编程细胞死亡. 这种真菌毒性机制涉及操纵植物中的NbIAA29和小麦中的TaIAA14,帮助病原体感染.

科学领域:

植物病理学植物病理学
分子植物-微生物相互作用
菌效应生物学的生物学

背景情况:

病原体分泌作用因子,以抑制宿主编程细胞死亡 (过敏反应),以成功殖民.
用真菌效应剂操纵宿主细胞死亡途径的精确机制尚未完全理解.

研究的目的:

研究Puccinia striiformis效应物PNPi在抑制宿主细胞死亡中的作用.
阐明PNPi介导的毒性背后的分子机制及其与宿主因素的相互作用.

主要方法:

使用BAX诱导的细胞死亡试验,对尼科蒂亚娜本塔米亚纳中的PNPi效应物的功能分析.
用RNA测序来识别受PNPi影响的宿主基因.
外源性奥克辛的应用和基因沉默 (NbIAA29) 来评估奥克辛的信号参与.
在小麦中的体内相互作用研究和病毒诱导的基因沉默 (TaIAA14).

主要成果:

在N. benthamiana中,PNPi抑制了BAX诱导的细胞死亡,其FtsN域介导.
PNPi降低了对auxin响应基因NbIAA29的调节,外源性auxin部分恢复了细胞死亡.
PNPi与小麦TaIAA14相互作用,其敲击增强了真菌发育并减少了小麦细胞死亡.

结论:

P. striiformis效应器PNPi通过向辅酶信号来抑制宿主细胞的死亡,特别是与NbIAA29/TaIAA14相互作用.
这种效应体与宿主之间的相互作用促进了真菌感染和殖民.
研究结果提供了关于真菌毒性策略和疾病控制的潜在目标的见解.

关键词:

在 PNPi 的情况下,PNPi 是艾克辛信号传输系统提供信号.细胞死亡是细胞死亡.效应器效应器是一个有效的效应器.条纹生条纹生

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