在Poloxamer 407水凝中平衡杀菌和功能性质:比较热和辐射技术
Angela De Lauretis1,2, Anne Eriksson Agger1, Antara Pal3,4
1Department of Biomaterials, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, 0455 Oslo, Norway.
Regenerative biomaterials
|February 21, 2025
概括
电子束 (e-beam) 辐射是Poloxamer 407水凝的最有效的灭菌方法. 这种方法保留了关键的凝和结构性质,与热或玛辐射不同,因此非常适合生物医学应用.
科学领域:
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
- 聚合物化学 聚合物化学
背景情况:
- 波洛克萨默407 (Pluronic F127) 是一种热敏水凝,在化品,生物医学和制药应用中具有显著的潜力.
- 绝育对于临床使用至关重要,但它可能会损害水凝的功能.
- 研究绝育方法对于保持Poloxamer 407的特性至关重要.
研究的目的:
- 为了评估各种灭菌技术对30%重/重Poloxamer 407水凝的影响.
- 确定最佳的灭菌方法,以保持水凝的功能和结构完整性.
主要方法:
- 绝育方法包括蒸汽热,干热,玛辐射和电子束 (e-beam) 辐射.
- 分析包括评估凝性质,pH值,光谱,色谱,X射线散射,风湿学,胀和细胞毒性 (LDH释放).
主要成果:
- 热杀菌改变了凝和结构性质,由于水蒸发和氧化,干热特别有害.
- 马辐射导致由于氧化降解而产生酸性和细胞毒性水凝.
- 电子束辐射 (15-25 kGy) 有效地保持了弹性,凝性和结构性质,同时提高了机械弹性和缓解胀.
结论:
- 以15-25kGy的E射线辐射是30%w/vPoloxamer 407水凝的最合适的灭菌方法.
- 这种方法保持了生物医学应用的必不可少的水凝特性.
- 其他方法,如热和玛辐射,会对水凝的性能和安全产生负面影响.
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