Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Toward scalable species descriptions for dark taxa: the role of molecular data.

ZooKeys·2026
Same author

Constructing a lower-bound estimate of the global number of insect species on a hyperdiverse empirical foundation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

BarcodeBERT: transformers for biodiversity analyses.

Bioinformatics advances·2026
Same author

Metabarcoding of larval guts reveals diet diversity in native apex predators-the yellowjacket wasps <i>Vespula vulgaris</i> and <i>Vespula germanica</i>.

Insectes sociaux·2026
Same author

Globally aggregated biodiversity data impact predictive and descriptive research.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

BOLDistilled: Automated Construction of Comprehensive but Compact DNA Barcode Reference Libraries.

Molecular ecology resources·2025

相关实验视频

Updated: Jun 27, 2026

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose
12:11

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose

Published on: November 13, 2013

15.3K

使用metabarcoding方法识别潜在的昆虫生态相互作用.

Nicole D Borsato1, Katherine Lunn1, Nina R Garrett1

  • 1Biology, York University, Toronto, ON, Canada.

PeerJ
|February 21, 2025
PubMed
概括

分子方法通过分析DNA痕迹来揭示神秘的物种相互作用. 这项研究比较了害虫和授粉者的标记物,为关节动物研究提供了通用的协议.

关键词:
DNA 条形码编码昆虫 昆虫是一种昆虫.超标标的代码化种类的相互作用 种类的相互作用

更多相关视频

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health
08:36

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

Published on: October 9, 2017

9.7K
A Rapid Method to Confine and Safely Handle Bees in the Field
03:45

A Rapid Method to Confine and Safely Handle Bees in the Field

Published on: August 23, 2024

731

相关实验视频

Last Updated: Jun 27, 2026

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose
12:11

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose

Published on: November 13, 2013

15.3K
Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health
08:36

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

Published on: October 9, 2017

9.7K
A Rapid Method to Confine and Safely Handle Bees in the Field
03:45

A Rapid Method to Confine and Safely Handle Bees in the Field

Published on: August 23, 2024

731

科学领域:

  • 生态生态学 生态生态学
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 量化神秘物种相互作用是很困难的,特别是对于微生物或形态学上具有挑战性的类型.
  • 像DNA测序这样的分子方法提供了强大的工具,可以通过环境DNA (eDNA) 来检测物种相互作用.
  • 降低测序成本使大规模分析成为可能,将重点转移到为广泛的生物见解选择最佳标记物.

研究的目的:

  • 为了比较分子标记物及其分析方法在模型关节动物群体的不同生命阶段.
  • 开发和评估用于识别涉及生态和经济重要害虫 (海绵,灰) 和授粉者 (蜜蜂,黄蜂) 的相互作用的协议.
  • 建立适用于各种生态系统的关节动物宿主种群的通用方法.

主要方法:

  • 使用了Illumina MiSeq和牛津纳米孔MinION测序平台.
  • 评估了7对针对5个分子标记物的原始组:植物,真菌,微生物,昆虫和线虫.
  • 应用方法模拟关节动物群,包括害虫物种和蜜蜂酒店中的授粉者.

主要成果:

  • 确定了数百种潜在的生态相互作用.
  • 建立了适用于各种节肢动物种群的通用分子方法.
  • 提供了根据研究系统选择合适的分子标记物的建议.

结论:

  • 分子标记器有效地揭示了神秘的物种相互作用,提供了宝贵的生态数据.
  • 一般化的方法和标记者的建议可以推进大规模的关节动物相互作用研究.
  • 从背景eDNA污染区分真正的生态相互作用仍然是一个重大挑战.