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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: May 27, 2025

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Published on: March 12, 2015

用金属复合体进行超分辨率显微镜的进步:用于纳米尺度可视化的功能成像剂.

Nur Aininie Yusoh¹, Martin R Gill², Xiaohe Tian¹

¹Department of Radiology, Huaxi MR Research Center (HMRRC), Institution of Radiology and Medical Imaging, West China Hospital of Sichuan University, Sichuan University, Chengdu, Sichuan, China. xiaohe.t@wchscu.cn.

Chemical Society reviews

|February 21, 2025

概括

金属复合物为高级超分辨率显微镜 (SRM) 提供了卓越的光稳定性和可调节的发光. 这些探测器能够进行深层组织成像和超神应用,克服有机光探测器的局限性.

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Last Updated: May 27, 2025

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科学领域:

生物成像是一种生物成像.
纳米技术纳米技术
材料科学材料科学材料科学

背景情况:

超分辨率显微镜 (SRM) 提供纳米尺度可视化,但受有机探针的光漂白和不稳定性限制.
现有的探头在深层组织成像方面表现不佳,限制了生物过程的观察.
金属复合物提供了增强的光稳定性,可调节的发光和长时间的激发状态寿命.

研究的目的:

审查最近在超分辨率显微镜中应用金属复合物的进展.
突出金属复合体在可视化亚细胞结构和活细胞动态中的实用性.
讨论金属复合体在疾病诊断和治疗中的治疗潜力.

主要方法:

探索金属复合物的合理设计策略,以提高生物相容性和特异性.
讨论用于SRM应用的金属纳米粒子 (NP) 和混合纳米尺度探针.
在深层组织透和多重成像中分析金属复合物的性能.

主要成果:

金属复合物克服了与有机探针相关的光漂白和光稳定性问题.
这些复杂物使得精确的亚细胞准,长期成像和复杂结构的可视化.
金属复合物促进实时诊断和治疗,显示疾病的治疗潜力.

结论:

金属复合物是推进超分辨率显微镜的多功能工具.
理性设计优化金属复合物,以提高生物相容性,特异性和深层组织成像.
新兴的混合探测器为克服当前SRM限制提供了有希望的解决方案.