Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Enhancement of hidden Markov model analyses for improved inference of archaic introgression in modern humans.

Molecular biology and evolution·2026
Same author

Establishment of DNA methylation during primate germ cell development.

Nature communications·2026
Same author

In Situ Expression of miR-371a-3p and miR-373-3p in Testicular Germ Cell Tumours and Detection in Serum.

Andrology·2026
Same author

Long-read sequencing of primate testis and human sperm allows identification of recombination events in individuals.

Nature communications·2025
Same author

Cross-dataset pan-cancer detection by correlating cell-free DNA fragment coverage with open chromatin sites across cell types.

Nature communications·2025
Same author

Changes in Circulating Levels of miR-30b During Minipuberty and Puberty in Girls.

The Journal of clinical endocrinology and metabolism·2025

相关实验视频

Updated: Jun 7, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

11.6K

从PacBio HiFi数据估计基因转换通道的长度和速率

Anders Poulsen Charmouh1, Peter Sørud Porsborg1, Lasse Thorup Hansen2

  • 1Bioinformatics Research Centre, Aarhus University, University City 81, DK-8000 Aarhus C, Denmark.

Molecular biology and evolution
|February 21, 2025
PubMed
概括

估计基因转化率和通道长度是很困难的. 一种使用长期读取的测序数据的新方法可以准确地测量这些因素,即使基因变异很小,也可以应用于人类精子数据.

关键词:
基因转换 基因转换基因组的演化 基因组的演化基因组学方法 基因组学方法没有交叉的非交叉.再组合的复合方式.

更多相关视频

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.2K
ATAC-Seq Optimization for Cancer Epigenetics Research
07:13

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

4.1K

相关实验视频

Last Updated: Jun 7, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

11.6K
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.2K
ATAC-Seq Optimization for Cancer Epigenetics Research
07:13

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

4.1K

科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 基因转换是一种关键的遗传重组过程,涉及DNA序列转移.
  • 它在等位基因组合和突变逆转中发挥作用,但准确的速率和通道长度估计仍然具有挑战性.
  • 基因转换是非交叉 (NCO) 事件的一个子集.

研究的目的:

  • 开发和验证一种用于估计基因转换率,通道长度和检测概率的新方法.
  • 为了能够从高保真性 (HiFi) PacBio 长时间读取的测序数据,特别是从子细胞的测序数据中推断.
  • 提供一种无偏见的方法,即使在低单核酸变异 (SNV) 密度下也能有效运行.

主要方法:

  • 开发一种新的计算方法来分析长时间读取的测序数据.
  • 使用模拟的数据集与不同的通道长度,NCO事件数量,SNV密度和SNV密度相关性.
  • 该方法应用于来自人类精子的PacBio测序数据.

主要成果:

  • 这种新方法准确地估计了基因转换通道的长度,速率和检测概率.
  • 该方法在不同的SNV密度中是无偏见的,不需要人口或进化假设.
  • 模拟表明,在较低的SNV密度下 (例如,人类),只有约2%的NCO事件可以被检测为基因转换.

结论:

  • 提出的方法提供了一个强大的方法来量化基因转换事件使用长读序列.
  • 这种工具对于研究包括人类在内的各种生物体中的遗传变异和重组是有价值的.
  • 这些发现凸显了低变异基因组中基因转换事件的低可检测性.