由几种植物Toll/interleukin-1受体含有免疫蛋白诱导的下游信号在升高的温度下是稳定的
Héloïse Demont1, Céline Remblière1, Raphaël Culerrier1
1Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE, CNRS, 31326 Castanet-Tolosan, France.
Cell reports
|February 21, 2025
概括
通过核酸结合的氨酸丰富的重复受体 (NLRs) 介导的植物免疫反应在高温下减弱. 这项研究揭示了单独的Toll/interleukin-1受体 (TIR) 域在热应激下保持信号传输,与全长NLR不同.
科学领域:
- 植物生物学 植物生物学
- 分子免疫学分子免疫学
- 植物病原体相互作用
背景情况:
- 植物免疫力,特别是由核酸结合的氨酸丰富重复受体 (NLRs) 介导的反应,在最佳生长温度之外经常被抑制.
- 这种取决于温度的植物免疫反应减弱背后的分子机制尚未完全理解.
- 众所周知,N端的Toll/互白素-1受体 (TIR) 域足以启动免疫信号传递.
研究的目的:
- 研究植物NLR介导免疫的温度依赖调节的分子机制.
- 为了确定单独的Toll/interleukin-1受体 (TIR) 域是否在触发免疫信号时,与全长NLR相比,具有不同的温度敏感性.
- 了解气候变化对植物疾病耐药性的影响.
主要方法:
- 条件激活两个具有良好特征的TIR含有NLRs (TNLs) 和它们在Arabidopsis中孤立的TIR域.
- 下游信号通路的分析,包括增强的疾病易感性1 (EDS1) /助手NLRs与RPW8类似的N终端CCR域 (RNL) 要求和酸通路激活.
- 在允许温度和高温 (30°C) 的信号通路维护的比较.
主要成果:
- 在允许的温度下,全长的TNL及其孤立的TIR域触发了相同的信号通路.
- 这种信号通路,当单独由TIR域诱导时,在高温 (30°C) 中保持.
- 然而,在高温下,全长TNL引发的信号无法维持.
结论:
- TIR域具有内在的特性,允许在更高的温度下持续免疫信号,独立于完整的NLR结构.
- 温度显著影响全长NLR的功能,这表明一个关键的监管机制.
- 进一步研究温度对NLRs的影响对于在全球变暖的背景下增强植物抗病能力至关重要.
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