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从纳米集群产生的内源性自由电子参与的无核活性剂的电化学发光及其免疫测试应用

Yaojia Ai¹, Xuwen Gao¹, Xiaowen Xu¹

¹School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.

Analytical chemistry

|February 21, 2025

概括

这项研究引入了一种新的无核心活性剂电化学发光 (ECL) 免疫试验,使用含硫空缺的黄金纳米集群. 这种方法简化了程序,提高了检测癌胚抗原等生物标志物的性能.

科学领域:

纳米材料科学科学纳米材料科学
分析化学分析化学
生物医学工程生物医学工程

背景情况:

商业电化学发光 (ECL) 免疫测试通常需要一个核心活性剂,并在+1.40V (vs Ag/AgCl) 工作.
外源核心活性剂可以引入变异性并复杂化测试程序.
开发无核活性剂的ECL系统对于简化和强大的免疫测试是可取的.

研究的目的:

为无核活性剂ECL免疫试验提出一种新的含硫空缺和自由电子策略.
为了利用金纳米集群 (AuNCs) 作为阳极电化学发光体.
展示该策略用于检测人类癌胚抗原 (CEA) 的应用.

主要方法:

合成含有硫空缺的氨酸覆盖金纳米集群 (Met-AuNCs).
使用电子磁共振 (EPR) 调查Met-AuNC中的富电子性质和内源性自由电子.
通过在阳极 (+0.86 V) 氧化Met-AuNCs来开发一种无核心活性剂的ECL免疫试验.

主要成果:

由于缺乏协调,Met-AuNCs表现出一种涉及硫空缺的,富含电子的性质.
在Met-AuNCs中的内源性自由电子通过与外源性孔直接结合来促进光辐射.
无核活性剂的ECL免疫试验成功量化了人类癌胚抗原 (CEA) 从10到5000 pg/mL,检测极限为5 pg/mL.

结论:

成功开发了一种基于AuNC中的硫空缺和自由电子的新型无核活性剂ECL免疫测试策略.
这种方法通过消除对外部核心活性剂的需求,简化了ECL免疫测试操作.
该战略显示,对于设计先进的纳米电化学光体来提高免疫测试性能,具有显著的前景.

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