布里奥菲勒姆皮纳图姆 (Bryophyllum pinnatum) 调节了与人类肌胎细胞中早产相关的信号通路
Leonie Zurfluh1, Stefanie Santos2, Annina Ruppen3
1Department of Obstetrics, University Hospital Zurich, Zurich, Switzerland; University of Zurich, Zurich, Switzerland.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
|February 21, 2025
概括
布里奥菲勒姆皮纳图姆 (Bryophyllum pinnatum) 叶汁通过抑制催产素诱导的信号和炎症,有效治疗早产. 果汁中的特定布法迪化物可以减少关键的炎症标志物和子宫收缩,而不会产生细胞毒性.
科学领域:
- 药理学 药理学是指药理学的学科.
- 产科 产科 产科 产科 产科
- 细胞生物学 细胞生物学
背景情况:
- Bryophyllum pinnatum的制剂已被公认为用于治疗早产.
- 以前的研究表明,B. pinnatum抑制了催产素诱导的细胞内 (Ca2+) 信号和肌肉细胞中的炎症.
- 造成这些影响的特定化合物和途径仍然未被确定.
研究的目的:
- 确定B. pinnatum中活性化合物,它们负责抑制肌肉细胞中催产素诱导的影响.
- 为了研究涉及B. pinnatum的毒解活动的潜在信号通路.
- 评估B. pinnatum对子宫收缩性和细胞外矩阵蛋白的影响.
主要方法:
- 在体外研究中,使用人类肌肉细胞 (hTERT-C3) 与标记蓝色测定,Ca2+成像,ELISA,蛋白质组学和实时PCR.
- 使用人体肌肉体器官浴模型的ex vivo收缩性研究.
- 从B. pinnatum叶压汁中分析个别的bufadienolides和丰富的部分.
主要成果:
- 没有任何一种化合物能够复制整个叶汁对催产素诱导的Ca2+信号的抑制作用.
- 一个富含布法迪埃诺利德的部分和特定的布法迪埃诺利德 (贝萨尔德基因-1,3,5-乙酸盐,布里奥菲林A,贝萨尔德基因-3-乙酸盐) 抑制了催产素诱导的COX-2表达和NFκB激活.
- B. pinnatum 果汁抑制了前列腺素 F2α 诱导的子宫肌收缩和减少了细胞外矩阵蛋白质的表达.
- 抑制作用不是由于细胞毒性造成的.
结论:
- 在B. pinnatum中,特定的bufadienolides通过调节炎症路径和收缩性来促进其毒解作用.
- B. pinnatum显示出作为一种耐受良好,长期治疗早产的潜力.
- 对B. pinnatum的活性化合物和机制进行进一步研究是有必要的.
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