多种机制通过增加poxtA基因表达来驱动临床Enterococcus faecium分离物中的linezolid耐药性
Fernando Lázaro-Perona1, Paula Navarro-Carrera1, Iván Bloise1
1Servicio de Microbiología y Parasitología Clínica, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain.
Journal of global antimicrobial resistance
|February 21, 2025
概括
Enterococcus faecium中的poxtA基因并不总是意味着linezolid耐药性,但它是一个危险因素. 对poxtA基因载体的分子监测可以帮助预防耐药性传播.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- poxtA基因通过编码ATP结合盒F蛋白来赋予可转移的linezolid抗性.
- 在Enterococcus faecium中,poxtA基因的存在并不总是与linezolid耐药性相关.
- 了解影响poxtA阳性分离物中linezolid敏感性的因素至关重要.
研究的目的:
- 为了研究poxtA基因在临床Enterococcus faecium分离物中对linezolid敏感性的作用.
- 分析与poxtA阳性菌株中linezolid耐药性相关的遗传和蛋白质组因素.
主要方法:
- 五个poxtA阳性临床Enterococcus faecium分离物的全基因组测序 (短和长读).
- 基因组组装以确定poxtA基因位置和相关的遗传元素.
- 蛋白质组学用于量化PoxtA蛋白水平,并与耐药性表型相关联.
主要成果:
- 在三种分离物中,对线化物的耐药性与poxtA基因促销器删除,核糖体蛋白L4突变,并列重复或增加等离子体拷贝数量有关.
- 蛋白质组学分析证实了PoxtA蛋白质的存在和在耐药菌株中的高表达.
- 在分离物中观察到的最高PoxtA表达与促进物删除.
结论:
- poxtA基因是Enterococcus faecium中linezolid耐药性发展的重要危险因素,尽管不是一个确定的标记.
- 建议对肠道poxtA基因载体进行分子监测,以防止linezolid耐药性传播.
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