在Apostichopus japonicus中,AJMALT1通过NF-κB通路促进了Vibrio splendidus诱导的炎症

相关实验视频

Updated: May 26, 2025

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

Haiping Liu¹, Jiping Wang¹, Weiwei Zhang¹

¹Key Laboratory of Aquacultural Biotechnology (Ningbo University), Ministry of Education, Ningbo, 315800, PR China.

Developmental and comparative immunology

|February 21, 2025

概括

海中的粘膜相关淋巴组织淋巴瘤转位蛋白1 (MALT1) 加剧了炎症. 沉默AJMALT1降低了炎症反应和细胞因子表达,表明它在免疫路径中的作用.

科学领域:

海洋生物学海洋生物学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

粘膜关联淋巴组织淋巴瘤转位蛋白1 (MALT1) 是炎症反应中的关键信号分子.
了解MALT1在无脊椎动物免疫力中的作用对于海洋动物健康至关重要.

研究的目的:

为了研究海Apostichopus japonicus中的AJMALT1的免疫功能.
为了阐明A. japonicus中AJMALT1介导的炎症背后的分子机制.

主要方法:

使用转录组数据和RACE技术克隆完整长度的AjMALT1基因.
对AJMALT1,AJIL17和AJNLRP3的定量基因表达分析.
通过RNA干扰 (siRNA) 来抑制AJMALT1的表达.
肠道组织的组织学检查.
对AjTRAF6和AjRel表达和核转位的分析.

主要成果:

AjMALT1在A. japonicus的所有组织中表达,在胆红细胞和肠道中含量较高.
在Vibrio splendidus感染期间,AjMALT1的表达上调,与促炎性细胞因子AjIL17和AjNLRP3相关.
沉默AJMALT1会降低AJIL17和AJNLRP3的表达,缓解肠道炎症,并抑制AJTRAF6依赖的NF-κB通路.

结论:

在A. japonicus.中,AJMALT1在加剧肠道和肠膜炎症方面发挥着重要作用.
依赖AJTRAF6的NF-κB通路与AJMALT1介导的炎症反应有关.
AjMALT1是管理水产养殖中的炎症性疾病的潜在目标.

关键词:

亚波斯蒂科普斯 (Apostichopus japonicus) 是一个日本动物.免疫反应的免疫反应.炎症炎症是一种炎症.马耳他语马耳他语这种病毒叫做Vibrio splendidus.

更多相关视频

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

相关概念视频

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

Inflammation

Inflammation

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...